Cardiovascular Antibodies
Anti-MFGE8 Antibody (CAB12322)
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- SKU:
- CAB12322
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cardiovascular
Description
| Antibody Name: | Anti-MFGE8 Antibody |
| Antibody SKU: | CAB12322 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human MFGE8 (NP_001108086.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:100 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse heart, Rat heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human MFGE8 (NP_001108086.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MPRP RLLA ALCG ALLC APSL LVAL DICS KNPC HNGG LCEE ISQE VRGD VFPS YTCT CLKG YAGN HCET KCVE PLGL ENGN IANS QIAA SSVR VTFL GLQH WVPE LARL NRAG MVNA WTPS SNDD NPWI QVNL LRRM WVTG VVTQ GASR LASH EYLK AFKV AYSL NGHE FDFI HDVN KKHK EFVG NWNK NAVH VNLF ETPV |
| Gene ID: | 4240 |
| Uniprot: | Q08431 |
| Cellular Location: | Membrane, Peripheral membrane protein, Secreted |
| Calculated MW: | 35kDa/37kDa/43kDa |
| Observed MW: | 54kDa |
| Synonyms: | MFGE8, BA46, EDIL1, HMFG, HsT19888, MFG-E8, MFGM, OAcGD3S, SED1, SPAG10, hP47 |
| Background: | This gene encodes a preproprotein that is proteolytically processed to form multiple protein products. The major encoded protein product, lactadherin, is a membrane glycoprotein that promotes phagocytosis of apoptotic cells. This protein has also been implicated in wound healing, autoimmune disease, and cancer. Lactadherin can be further processed to form a smaller cleavage product, medin, which comprises the major protein component of aortic medial amyloid (AMA). Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | MFGE8: Plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. Promotes VEGF-dependent neovascularization. Contributes to phagocytic removal of apoptotic cells in many tissues. Specific ligand for the alpha-v/beta-3 and alpha-v/beta-5 receptors. Also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner. Zona pellucida-binding protein which may play a role in gamete interaction. Binds specifically to rotavirus and inhibits its replication. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 15q25 Cellular Component: extrinsic to plasma membrane; extracellular matrix; extracellular space; membrane; extracellular region; vesicle; external side of plasma membrane Molecular Function:integrin binding; phosphatidylethanolamine binding; phosphatidylserine binding Biological Process: viral reproduction; single fertilization; phagocytosis, recognition; angiogenesis; phagocytosis, engulfment; cell adhesion |
| UniProt Code: | Q08431 |
| NCBI GenInfo Identifier: | 2506380 |
| NCBI Gene ID: | |
| NCBI Accession: | Q08431.2 |
| Molecular Weight: | |
| NCBI Full Name: | Lactadherin |
| UniProt Protein Name: | Lactadherin |
| UniProt Synonym Protein Names: | Breast epithelial antigen BA46; HMFG; MFGM; Milk fat globule-EGF factor 8; MFG-E8; SED1Cleaved into the following 2 chains:Lactadherin short form; Medin |
| UniProt Gene Name: | MFGE8 |
| UniProt Entry Name: | MFGM_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MFGE8 antibody (CAB12322) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
 | Immunofluorescence analysis of C6 cells using MFGE8 antibody (CAB12322) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of L929 cells using MFGE8 antibody (CAB12322) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U-2 OS cells using MFGE8 antibody (CAB12322) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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