Cell Biology Antibodies 6
Anti-METTL16 Antibody (CAB15894)
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- SKU:
- CAB15894
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-METTL16 Antibody |
| Antibody SKU: | CAB15894 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 263-562 of human METTL16 (NP_076991.3). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-251MG, HeLa |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 263-562 of human METTL16 (NP_076991.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | RIQG VPKV TYTE FCQG RTMR WALA WSFY DDVT VPSP PSKR RKLE KPRK PITF VVLA SVMK ELSL KASP LRSE TAEG IVVV TTWI EKIL TDLK VQHK RVPC GKEE VSLF LTAI ENSW IHLR RKKR ERVR QLRE VPRA PEDV IQAL EEKK PTPK ESGN SQEL ARGP QERT PCGP ALRE GEAA AVEG PCPS QESL SQEE NPEP TEDE RSEE KGGV EVLE SCQG SSNG AQDQ EASE QFGS PVAE RGKR LPGV AGQY LFKC LINV KKEV DDAL VEMH WVEG QNRD LMNQ LCTY IRNQ IFRL VAVN |
| Gene ID: | 79066 |
| Uniprot: | Q86W50 |
| Cellular Location: | |
| Calculated MW: | 25kDa/63kDa |
| Observed MW: | 80kDa |
| Synonyms: | METTL16, METT10D |
| Background: |
| UniProt Protein Function: | MGC3329: Probable methyltransferase. Belongs to the methyltransferase superfamily. METTL16/RlmF family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Methyltransferase; EC 2.1.1.- Chromosomal Location of Human Ortholog: 17p13.3 Molecular Function:methyltransferase activity Biological Process: methylation |
| UniProt Code: | Q86W50 |
| NCBI GenInfo Identifier: | 269849619 |
| NCBI Gene ID: | 79066 |
| NCBI Accession: | Q86W50.2 |
| UniProt Secondary Accession: | Q86W50,Q86TE5, Q96T16, Q9BVG7, D3DTI8, |
| UniProt Related Accession: | Q86W50 |
| Molecular Weight: | 562 |
| NCBI Full Name: | Methyltransferase-like protein 16 |
| NCBI Synonym Full Names: | methyltransferase like 16 |
| NCBI Official Symbol: | METTL16 |
| NCBI Official Synonym Symbols: | METT10D |
| NCBI Protein Information: | methyltransferase-like protein 16; putative methyltransferase METT10D; methyltransferase 10 domain containing; methyltransferase 10 domain-containing protein |
| UniProt Protein Name: | Methyltransferase-like protein 16 |
| UniProt Synonym Protein Names: | Methyltransferase 10 domain-containing protein |
| Protein Family: | Methyltransferase-like protein |
| UniProt Gene Name: | METTL16 |
| UniProt Entry Name: | MET16_HUMAN |
 | Western blot analysis of extracts of various cell lines, using METTL16 antibody (CAB15894) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunofluorescence analysis of C6 cells using METTL16 antibody (CAB15894) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of NIH/3T3 cells using METTL16 antibody (CAB15894) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U-2 OS cells using METTL16 antibody (CAB15894) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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