Developmental Biology

Anti-METTL14 Antibody (CAB8530)

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SKU:
CAB8530
Product Type:
Antibody
Applications:
WB
Applications:
IHC
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Developmental Biology

Description

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Antibody Name:Anti-METTL14 Antibody
Antibody SKU:CAB8530
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human METTL14 (NP_066012.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:A-549, Raji, Mouse brain, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human METTL14 (NP_066012.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MDSR LQEI RERQ KLRR QLLA QQLG AESA DSIG AVLN SKDE QREI AETR ETCR ASYD TSAP NAKR KYLD EGET DEDK MEEY KDEL EMQQ DEEN LPYE EEIY KDSS TFLK GTQS LNPH NDYC QHFV DTGH RPQN FIRD VGLA DRFE EYPK LREL IRLK DELI AKSN TPPM YLQA DIEA FDIR ELTP KFDV ILLE PPLE EYYR ETGI TANE KC
Gene ID:57721
Uniprot:Q9HCE5
Cellular Location:Nucleus
Calculated MW:52kDa
Observed MW:60KDa
Synonyms:METTL14, hMETTL14
Background:
UniProt Protein Function:The METTL3-METTL14 heterodimer forms a N6-methyltransferase complex that methylates adenosine residues at the N6 position of some mRNAs and regulates the circadian clock, differentiation of embryonic stem cells and cortical neurogenesis (PubMed:24316715, PubMed:24407421, PubMed:25719671, PubMed:27373337, PubMed:27281194). In the heterodimer formed with METTL3, METTL14 constitutes the RNA-binding scaffold that recognizes the substrate rather than the catalytic core (PubMed:27627798, PubMed:27373337, PubMed:27281194). N6-methyladenosine (m6A), which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in mRNA stability and processing (PubMed:24316715, PubMed:24407421, PubMed:25719671). M6A acts as a key regulator of mRNA stability by promoting mRNA destabilization and degradation. In embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naive pluripotency-promoting transcripts results in transcript destabilization. M6A regulates spermatogonial differentiation and meiosis and is essential for male fertility and spermatogenesis. M6A also regulates cortical neurogenesis: m6A methylation of transcripts related to transcription factors, neural stem cells, the cell cycle and neuronal differentiation during brain development promotes their destabilization and decay, promoting differentiation of radial glial cells.
UniProt Code:Q9HCE5
NCBI GenInfo Identifier:24308265
NCBI Gene ID:57721
NCBI Accession:NP_066012.1
UniProt Secondary Accession:Q9HCE5,Q969V2, A6NIG1,
UniProt Related Accession:Q9HCE5
Molecular Weight:52150
NCBI Full Name:N6-adenosine-methyltransferase non-catalytic subunit
NCBI Synonym Full Names:methyltransferase like 14
NCBI Official Symbol:METTL14  
NCBI Official Synonym Symbols:hMETTL14  
NCBI Protein Information:N6-adenosine-methyltransferase non-catalytic subunit; N6-adenosine-methyltransferase subunit METTL14
UniProt Protein Name:N6-adenosine-methyltransferase non-catalytic subunit
UniProt Synonym Protein Names:Methyltransferase-like protein 14
Protein Family:N6-adenosine-methyltransferase
UniProt Gene Name:METTL14  
Anti-METTL14 Polyclonal Antibody (CAB8530)Western blot analysis of extracts of various cell lines, using METTL14 antibody (CAB8530) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-METTL14 Polyclonal Antibody (CAB8530)Western blot analysis of extracts of various cell lines, using METTL14 antibody (CAB8530) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-METTL14 Polyclonal Antibody (CAB8530)Immunohistochemistry of paraffin-embedded Mouse brain using METTL14 antibody (CAB8530) at dilution of 1:100 (40x lens).
Anti-METTL14 Polyclonal Antibody (CAB8530)Immunohistochemistry of paraffin-embedded Rat spleen using METTL14 antibody (CAB8530) at dilution of 1:100 (40x lens).
Anti-METTL14 Polyclonal Antibody (CAB8530)Immunohistochemistry of paraffin-embedded Human mammary cancer using METTL14 antibody (CAB8530) at dilution of 1:100 (40x lens).
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