Cell Biology Antibodies 10
Anti-MEST Antibody (CAB6931)
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- SKU:
- CAB6931
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-MEST Antibody |
| Antibody SKU: | CAB6931 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 146-335 of human MEST (NP_002393.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse |
| Positive Samples: | MCF7, BT-474, HT-29, Mouse testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 146-335 of human MEST (NP_002393.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | HDYG DIVA QELL YRYK QNRS GRLT IKSL CLSN GGIF PETH RPLL LQKL LKDG GVLS PILT RLMN FFVF SRGL TPVF GPYT RPSE SELW DMWA GIRN NDGN LVID SLLQ YINQ RKKF RRRW VGAL ASVT IPIH FIYG PLDP VNPY PEFL ELYR KTLP RSTV SILD DHIS HYPQ LEDP MGFL NAYM GFIN SF |
| Gene ID: | 4232 |
| Uniprot: | Q5EB52 |
| Cellular Location: | Endoplasmic reticulum membrane, Multi-pass membrane protein |
| Calculated MW: | 33kDa/37kDa/38kDa |
| Observed MW: | 39kDa |
| Synonyms: | MEST, PEG1 |
| Background: | This gene encodes a member of the alpha/beta hydrolase superfamily. It is imprinted, exhibiting preferential expression from the paternal allele in fetal tissues, and isoform-specific imprinting in lymphocytes. The loss of imprinting of this gene has been linked to certain types of cancer and may be due to promotor switching. The encoded protein may play a role in development. Alternatively spliced transcript variants encoding multiple isoforms have been identified for this gene. Pseudogenes of this gene are located on the short arm of chromosomes 3 and 4, and the long arm of chromosomes 6 and 15. |
| UniProt Protein Function: | MEST: a member of the alpha/beta hydrolase superfamily. It is imprinted, exhibiting preferential expression from the paternal allele in fetal tissues, and isoform-specific imprinting in lymphocytes. The loss of imprinting of this gene has been linked to certain types of cancer and may be due to promotor switching. The encoded protein may play a role in development. Alternatively spliced transcript variants encoding multiple isoforms have been identified for this gene. Pseudogenes of this gene are located on the short arm of chromosomes 3 and 4, and the long arm of chromosomes 6 and 15. [provided by RefSeq, Dec 2011] |
| UniProt Protein Details: | Protein type:Membrane protein, multi-pass; Membrane protein, integral; EC 3.-.-.- Chromosomal Location of Human Ortholog: 7q32 Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum; integral to membrane Molecular Function:hydrolase activity Biological Process: response to retinoic acid; metabolic process; mesoderm development |
| UniProt Code: | Q5EB52 |
| NCBI GenInfo Identifier: | 145566794 |
| NCBI Gene ID: | |
| NCBI Accession: | Q5EB52.2 |
| Molecular Weight: | ~ 39kDa |
| NCBI Full Name: | Mesoderm-specific transcript homolog protein |
| UniProt Protein Name: | Mesoderm-specific transcript homolog protein |
| UniProt Synonym Protein Names: | Paternally-expressed gene 1 protein |
| Protein Family: | Mesoderm-specific transcript protein |
| UniProt Gene Name: | MEST |
| UniProt Entry Name: | MEST_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MEST antibody (CAB6931) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunofluorescence analysis of U2OS cells using MEST antibody (CAB6931). Blue: DAPI for nuclear staining. |
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