Immunology Antibodies 1
Anti-MEP1B Antibody (CAB10085)
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- SKU:
- CAB10085
- Product Type:
- Antibody
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Description
| Antibody Name: | Anti-MEP1B Antibody |
| Antibody SKU: | CAB10085 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 480-652 of human MEP1B (NP_005916.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:1000 |
| Reactivity: | Mouse |
| Positive Samples: | Mouse kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 480-652 of human MEP1B (NP_005916.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | ISGA NDDQ LQWP CPWQ QATM TLLD QNPD IRQR MSNQ RSIT TDPF MTTD NGNY FWDR PSKV GTVA LFSN GTQF RRGG GYGT SAFI THER LKSR DFIK GDDV YILL TVED ISHL NSTQ IQLT PAPS VQDL CSKT TCKN DGVC TVRD GKAE CRCQ SGED WWYM GERC EKRG STRD T |
| Gene ID: | 4225 |
| Uniprot: | Q16820 |
| Cellular Location: | Cell membrane, Secreted, Single-pass type I membrane protein |
| Calculated MW: | 79kDa |
| Observed MW: | 105kDa |
| Synonyms: | MEP1B |
| Background: | Meprins are multidomain zinc metalloproteases that are highly expressed in mammalian kidney and intestinal brush border membranes, and in leukocytes and certain cancer cells. They are involved in the hydrolysis of a variety of peptide and protein substrates, and have been implicated in cancer and intestinal inflammation. Mature meprins are oligomers of evolutionarily related, but separately encoded alpha and/or beta subunits. Homooligomers of alpha subunit are secreted, whereas, oligomers containing the beta subunit are plasma membrane-bound. This gene encodes the beta subunit. Targeted disruption of this gene in mice affects embryonic viability, renal gene expression profiles, and distribution of the membrane-associated alpha subunit in kidney and intestine. |
| UniProt Protein Function: | Membrane metallopeptidase that sheds many membrane-bound proteins. Exhibits a strong preference for acidic amino acids at the P1' position. Known substrates include: FGF19, VGFA, IL1B, IL18, procollagen I and III, E-cadherin, KLK7, gastrin, ADAM10, tenascin-C. The presence of several pro-inflammatory cytokine among substrates implicate MEP1B in inflammation. It is also involved in tissue remodeling due to its capability to degrade extracellular matrix components. |
| NCBI Summary: | Meprins are multidomain zinc metalloproteases that are highly expressed in mammalian kidney and intestinal brush border membranes, and in leukocytes and certain cancer cells. They are involved in the hydrolysis of a variety of peptide and protein substrates, and have been implicated in cancer and intestinal inflammation. Mature meprins are oligomers of evolutionarily related, but separately encoded alpha and/or beta subunits. Homooligomers of alpha subunit are secreted, whereas, oligomers containing the beta subunit are plasma membrane-bound. This gene encodes the beta subunit. Targeted disruption of this gene in mice affects embryonic viability, renal gene expression profiles, and distribution of the membrane-associated alpha subunit in kidney and intestine. [provided by RefSeq, Oct 2011] |
| UniProt Code: | Q16820 |
| NCBI GenInfo Identifier: | 815890920 |
| NCBI Gene ID: | 4225 |
| NCBI Accession: | NP_001295100.1 |
| UniProt Secondary Accession: | Q16820,Q670J1, B7ZM35, B9EGL6, |
| UniProt Related Accession: | Q16820 |
| Molecular Weight: | 79,571 Da |
| NCBI Full Name: | meprin A subunit beta isoform 2 |
| NCBI Synonym Full Names: | meprin A subunit beta |
| NCBI Official Symbol: | MEP1B |
| NCBI Protein Information: | meprin A subunit beta |
| UniProt Protein Name: | Meprin A subunit beta |
| UniProt Synonym Protein Names: | Endopeptidase-2; Meprin B; N-benzoyl-L-tyrosyl-P-amino-benzoic acid hydrolase subunit beta; PABA peptide hydrolase; PPH beta |
| Protein Family: | Meprin |
| UniProt Gene Name: | MEP1B |
 | Western blot analysis of extracts of mouse kidney, using MEP1B antibody (CAB10085) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s. |
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