Epigenetics & Nuclear Signaling Antibodies 2

Anti-MEF2B Antibody (CAB15987)

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SKU:
CAB15987
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-MEF2B Antibody
Antibody SKU:CAB15987
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MEF2B (NP_005910.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:SH-SY5Y
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MEF2B (NP_005910.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MGRK KIQI SRIL DQRN RQVT FTKR KFGL MKKA YELS VLCD CEIA LIIF NSAN RLFQ YAST DMDR VLLK YTEY SEPH ESRT NTDI LETL KRRG IGLD GPEL
Gene ID:100271849
Uniprot:Q02080
Cellular Location:Nucleus
Calculated MW:38kDa/39kDa
Observed MW:42kDa
Synonyms:MEF2B, RSRFR2
Background:The product of this gene is a member of the MADS/MEF2 family of DNA binding proteins. The protein is thought to regulate gene expression, including expression of the smooth muscle myosin heavy chain gene. This region undergoes considerable alternative splicing, with transcripts supporting two non-overlapping loci (GeneID 729991 and 100271849) as well as numerous read-through transcripts that span both loci (annotated as GeneID 4207). Several isoforms of this protein are expressed from either this locus or from some of the read-through transcripts annotated on GeneID 4207.
UniProt Protein Function:MEF2B: Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT](4)TAR-3', found in numerous muscle- specific genes. Activates transcription via this element. May be involved in muscle-specific and/or growth factor-related transcription. Belongs to the MEF2 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 19p13.11

Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; cell junction; nucleus

Molecular Function:protein dimerization activity; histone deacetylase binding; transcription factor activity

Biological Process: muscle development; transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter

NCBI Summary:This gene represents numerous read-through transcripts that span GeneID:729991 and 100271849. Many read-through transcripts are predicted to be nonsense-mediated decay (NMD) candidates, and are thought to be non-coding. Some transcripts are predicted to be capable of translation reinitiation at a downstream AUG, resulting in expression of at least one isoform of myocyte enhancer factor 2B (MEF2B) from this read-through locus. At least one additional MEF2B variant and isoform can be expressed from a downstream promoter, and is annotated on GeneID:100271849. [provided by RefSeq, Oct 2010]
UniProt Code:Q02080
NCBI GenInfo Identifier:5174543
NCBI Gene ID:4207
NCBI Accession:NP_005910
UniProt Related Accession:Q02080
Molecular Weight:39kDa
NCBI Full Name:myocyte-specific enhancer factor 2B isoform b
NCBI Synonym Full Names:BORCS8-MEF2B readthrough
NCBI Official Symbol:BORCS8-MEF2B  
NCBI Official Synonym Symbols:MEF2B; RSRFR2; MEF2BNB-MEF2B; LOC729991-MEF2B  
NCBI Protein Information:myocyte-specific enhancer factor 2B
UniProt Protein Name:Myocyte-specific enhancer factor 2B
UniProt Synonym Protein Names:RSRFR2; Serum response factor-like protein 2
Protein Family:Myocyte-specific enhancer factor
UniProt Gene Name:MEF2B  
UniProt Entry Name:MEF2B_HUMAN
Anti-MEF2B Antibody (CAB15987)Western blot analysis of extracts of SH-SY5Y cells, using MEF2B antibody (CAB15987) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3min.
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