Epigenetics & Nuclear Signaling Antibodies 2

Anti-MED7 Antibody (CAB16088)

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SKU:
CAB16088
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-MED7 Antibody
Antibody SKU:CAB16088
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-233 of human MED7 (NP_004261.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:293T, NIH/3T3, Mouse testis
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-233 of human MED7 (NP_004261.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MGEP QQVS ALPP PPMQ YIKE YTDE NIQE GLAP KPPP PIKD SYMM FGNQ FQCD DLII RPLE SQGI ERLH PMQF DHKK ELRK LNMS ILIN FLDL LDIL IRSP GSIK REEK LEDL KLLF VHVH HLIN EYRP HQAR ETLR VMME VQKR QRLE TAER FQKH LERV IEMI QNCL ASLP DDLP HSEA GMRV KTEP MDAD DSNN CTGQ NEHQ RENS GHRR DQII EKDA ALCV LIDE MNER P
Gene ID:9443
Uniprot:O43513
Cellular Location:Nucleus
Calculated MW:27kDa
Observed MW:27kDa
Synonyms:MED7, ARC34, CRSP33, CRSP9
Background:The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. Two transcript variants encoding the same protein have been found for this gene.
UniProt Protein Function:MED7: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Belongs to the Mediator complex subunit 7 family.
UniProt Protein Details:

Chromosomal Location of Human Ortholog: 5q33.3

Cellular Component: nucleoplasm; Srb-mediator complex; transcription factor complex

Molecular Function:protein binding; transcription coactivator activity

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter

NCBI Summary:The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:O43513
NCBI GenInfo Identifier:29337159
NCBI Gene ID:9443
NCBI Accession:O43513.1
UniProt Related Accession:O43513
Molecular Weight:27,245 Da
NCBI Full Name:Mediator of RNA polymerase II transcription subunit 7
NCBI Synonym Full Names:mediator complex subunit 7
NCBI Official Symbol:MED7  
NCBI Official Synonym Symbols:ARC34; CRSP9; CRSP33  
NCBI Protein Information:mediator of RNA polymerase II transcription subunit 7
UniProt Protein Name:Mediator of RNA polymerase II transcription subunit 7
UniProt Synonym Protein Names:Activator-recruited cofactor 34 kDa component; ARC34; Cofactor required for Sp1 transcriptional activation subunit 9; CRSP complex subunit 9; Mediator complex subunit 7; RNA polymerase transcriptional regulation mediator subunit 7 homolog; Transcriptional coactivator CRSP33
Protein Family:Putative mediator of RNA polymerase II transcription
UniProt Gene Name:MED7  
UniProt Entry Name:MED7_HUMAN
Anti-MED7 Antibody (CAB16088)Western blot analysis of extracts of various cell lines, using MED7 antibody (CAB16088) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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