Epigenetics & Nuclear Signaling Antibodies 4

Anti-MED6 Antibody (CAB8181)

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SKU:
CAB8181
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:MED6 Rabbit Polyclonal Antibody
Antibody SKU:CAB8181
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 27-246 of human MED6 (NP_005457.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:MCF7, Jurkat, HeLa
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 27-246 of human MED6 (NP_005457.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:GSVL DYFS ERSN PFYD RTCN NEVV KMQR LTLE HLNQ MVGI EYIL LHAQ EPIL FIIR KQQR QSPA QVIP LADY YIIA GVIY QAPD LGSV INSR VLTA VHGI QSAF DEAM SYCR YHPS KGYW WHFK DHEE QDKV RPKA KRKE EPSS IFQR QRVD ALLL DLRQ KFPP KFVQ LKPG EKPV PVDQ TKKE AEPI PETV KPEE KETT KNVQ QTVS AKGP PEKR MRLQ
Gene ID:10001
Uniprot:O75586
Cellular Location:Nucleus
Calculated MW:20kDa/28kDa/29kDa
Observed MW:28kDa
Synonyms:MED6, ARC33, NY-REN-28
Background:
UniProt Protein Function:MED6: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Belongs to the Mediator complex subunit 6 family.
UniProt Protein Details:

Protein type:Transcription factor

Chromosomal Location of Human Ortholog: 14q24.2

Cellular Component: membrane; nucleoplasm; nucleus; Srb-mediator complex

Molecular Function:protein binding; transcription coactivator activity; transcription factor binding

Biological Process: positive regulation of transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; transcriptional preinitiation complex assembly

UniProt Code:O75586
NCBI GenInfo Identifier:29840782
NCBI Gene ID:10001
NCBI Accession:O75586.2
UniProt Secondary Accession:O75586,O15401, Q53FE3, Q53HJ3, Q6FHQ4, Q9BTH1, Q9UHL1 B4DU17, B4E2P0,
UniProt Related Accession:O75586
Molecular Weight:20,859 Da
NCBI Full Name:Mediator of RNA polymerase II transcription subunit 6
NCBI Synonym Full Names:mediator complex subunit 6
NCBI Official Symbol:MED6  
NCBI Official Synonym Symbols:ARC33; NY-REN-28  
NCBI Protein Information:mediator of RNA polymerase II transcription subunit 6
UniProt Protein Name:Mediator of RNA polymerase II transcription subunit 6
UniProt Synonym Protein Names:Activator-recruited cofactor 33 kDa component; ARC33; Mediator complex subunit 6; hMed6; Renal carcinoma antigen NY-REN-28
Protein Family:Mediator of RNA polymerase II transcription
UniProt Gene Name:MED6  
UniProt Entry Name:MED6_HUMAN
Anti-MED6 Polyclonal Antibody (CAB8181)Western blot analysis of extracts of various cell lines, using MED6 antibody (CAB8181) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 45s.
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