Epigenetics & Nuclear Signaling Antibodies 4
Anti-MED4 Antibody (CAB9150)
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- SKU:
- CAB9150
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-MED4 Antibody |
| Antibody SKU: | CAB9150 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human MED4 (NP_054885.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:200 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | Jurkat, SH-SY5Y, HL-60, SW480, HT-1080 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human MED4 (NP_054885.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAAS SSGE KEKE RLGG GLGV AGGN STRE RLLS ALED LEVL SREL IEML AISR NQKL LQAG EENQ VLEL LIHR DGEF QELM KLAL NQGK IHHE MQVL EKEV EKRD SDIQ QLQK QLKE AEQI LATA VYQA KEKL KSIE KARK GAIS SEEI IKYA HRIS ASNA VCAP LTWV PGDP RRPY PTDL EMRS GLLG QMNN PSTN GVNG HLPG DALA AGRL PDVL APQY PWQS NDMS MNML PPNH SSDF LLEP PGHN KENE DDVE IMST DSSS SSSE SD |
| Gene ID: | 29079 |
| Uniprot: | Q9NPJ6 |
| Cellular Location: | Nucleus |
| Calculated MW: | 24kDa/29kDa |
| Observed MW: | 36kDa |
| Synonyms: | MED4, ARC36, DRIP36, HSPC126, TRAP36, VDRIP |
| Background: | This gene encodes a component of the Mediator complex. The Mediator complex interacts with DNA-binding gene-specific transcription factors to modulate transcription by RNA polymerase II. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. |
| UniProt Protein Function: | MED4: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Belongs to the Mediator complex subunit 4 family. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 13q14.2 Cellular Component: nucleoplasm; membrane; Srb-mediator complex; nucleus Molecular Function:protein binding; ligand-dependent nuclear receptor transcription coactivator activity; vitamin D receptor binding; transcription cofactor activity; receptor activity; thyroid hormone receptor binding Biological Process: steroid hormone receptor signaling pathway; regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; androgen receptor signaling pathway; gene expression |
| NCBI Summary: | This gene encodes a component of the Mediator complex. The Mediator complex interacts with DNA-binding gene-specific transcription factors to modulate transcription by RNA polymerase II. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012] |
| UniProt Code: | Q9NPJ6 |
| NCBI GenInfo Identifier: | 29840770 |
| NCBI Gene ID: | 29079 |
| NCBI Accession: | Q9NPJ6.1 |
| UniProt Secondary Accession: | Q9NPJ6,Q53GB4, Q53H68, Q5T912, Q6FHC4, Q6IA79, Q9BS95 Q9NYR5, B4DX67, |
| UniProt Related Accession: | Q9NPJ6 |
| Molecular Weight: | 24,947 Da |
| NCBI Full Name: | Mediator of RNA polymerase II transcription subunit 4 |
| NCBI Synonym Full Names: | mediator complex subunit 4 |
| NCBI Official Symbol: | MED4 |
| NCBI Official Synonym Symbols: | ARC36; VDRIP; DRIP36; TRAP36; HSPC126 |
| NCBI Protein Information: | mediator of RNA polymerase II transcription subunit 4; TRAP/SMCC/PC2 subunit p36; mediator, 34-kD subunit, homolog; activator-recruited cofactor 36 kDa component; vitamin D receptor-interacting protein, 36-kD; vitamin D3 receptor-interacting protein complex 36 kDa component |
| UniProt Protein Name: | Mediator of RNA polymerase II transcription subunit 4 |
| UniProt Synonym Protein Names: | Activator-recruited cofactor 36 kDa component; ARC36; Mediator complex subunit 4; TRAP/SMCC/PC2 subunit p36 subunit; Vitamin D3 receptor-interacting protein complex 36 kDa component; DRIP36 |
| Protein Family: | Mediator of RNA polymerase II transcription |
| UniProt Gene Name: | MED4 |
| UniProt Entry Name: | MED4_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MED4 antibody (CAB9150) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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