Epigenetics & Nuclear Signaling Antibodies 2
Anti-MED31 Antibody (CAB15831)
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- SKU:
- CAB15831
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-MED31 Antibody |
| Antibody SKU: | CAB15831 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-131 of human MED31 (NP_057144.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-87MG, Mouse brain, Mouse liver, Mouse testis, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-131 of human MED31 (NP_057144.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAAA VAME TDDA GNRL RFQL ELEF VQCL ANPN YLNF LAQR GYFK DKAF VNYL KYLL YWKD PEYA KYLK YPQC LHML ELLQ YEHF RKEL VNAQ CAKF IDEQ QILH WQHY SRKR MRLQ QALA EQQQ QNNT SGK |
| Gene ID: | 51003 |
| Uniprot: | Q9Y3C7 |
| Cellular Location: | Nucleus |
| Calculated MW: | 15kDa |
| Observed MW: | 16kDa |
| Synonyms: | MED31, 3110004H13Rik, CGI-125, Soh1 |
| Background: |
| UniProt Protein Function: | MED31: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Belongs to the Mediator complex subunit 31 family. |
| UniProt Protein Details: | Protein type:Transcription regulation Chromosomal Location of Human Ortholog: 17p13.1 Cellular Component: nucleoplasm; Srb-mediator complex Molecular Function:protein binding; protein complex binding Biological Process: limb development; regulation of transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; negative regulation of fibroblast proliferation; protein complex assembly; gene expression |
| UniProt Code: | Q9Y3C7 |
| NCBI GenInfo Identifier: | 7705592 |
| NCBI Gene ID: | 51003 |
| NCBI Accession: | NP_057144.1 |
| UniProt Secondary Accession: | Q9Y3C7,B2R4L9, |
| UniProt Related Accession: | Q9Y3C7 |
| Molecular Weight: | 15,805 Da |
| NCBI Full Name: | mediator of RNA polymerase II transcription subunit 31 |
| NCBI Synonym Full Names: | mediator complex subunit 31 |
| NCBI Official Symbol: | MED31 |
| NCBI Official Synonym Symbols: | Soh1; CGI-125; 3110004H13Rik |
| NCBI Protein Information: | mediator of RNA polymerase II transcription subunit 31; hSOH1; mediator complex subunit SOH1; mediator of RNA polymerase II transcription, subunit 31 homolog |
| UniProt Protein Name: | Mediator of RNA polymerase II transcription subunit 31 |
| UniProt Synonym Protein Names: | Mediator complex subunit 31; Mediator complex subunit SOH1; hSOH1 |
| Protein Family: | Mediator of RNA polymerase II transcription |
| UniProt Gene Name: | MED31 |
| UniProt Entry Name: | MED31_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MED31 antibody (CAB15831) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
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