Epigenetics & Nuclear Signaling Antibodies 1

Anti-MED30 Antibody (CAB13135)

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SKU:
CAB13135
Product Type:
Antibody
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-MED30 Antibody
Antibody SKU:CAB13135
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-178 of human MED30 (NP_542382.1).
Application:WB
Recommended Dilution:WB 1:1000 - 1:2000
Reactivity:Mouse
Positive Samples:Mouse brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-178 of human MED30 (NP_542382.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MSTP PLAA SGMA PGPF AGPQ AQQA AREV NTAS LCRI GQET VQDI VYRT MEIF QLLR NMQL PNGV TYHT GTYQ DRLT KLQD NLRQ LSVL FRKL RLVY DKCN ENCG GMDP IPVE QLIP YVEE DGSK NDDR AGPP RFAS EERR EIAE VNKK LKQK NQQL KQIM DQLR NLIW DINA MLAM RN
Gene ID:90390
Uniprot:Q96HR3
Cellular Location:Nucleus
Calculated MW:16kDa/20kDa
Observed MW:30kDa
Synonyms:MED30, MED30S, THRAP6, TRAP25
Background:
UniProt Protein Function:MED30: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Belongs to the Mediator complex subunit 30 family.
UniProt Protein Details:

Protein type:Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 8q24.11

Cellular Component: nucleoplasm; nucleus; Srb-mediator complex

Molecular Function:protein binding; receptor activity; thyroid hormone receptor binding; transcription cofactor activity

Biological Process: androgen receptor signaling pathway; positive regulation of transcription, DNA-dependent; steroid hormone receptor signaling pathway; transcription initiation from RNA polymerase II promoter

NCBI Summary:The multiprotein TRAP/Mediator complex facilitates gene expression through a wide variety of transcriptional activators. MED30 is a component of this complex that appears to be metazoan specific (Baek et al., 2002 [PubMed 11909976]).[supplied by OMIM, Nov 2010]
UniProt Code:Q96HR3
NCBI GenInfo Identifier:74731968
NCBI Gene ID:90390
NCBI Accession:Q96HR3.1
UniProt Secondary Accession:Q96HR3,C6GKU9,
UniProt Related Accession:Q96HR3
Molecular Weight:16,279 Da
NCBI Full Name:Mediator of RNA polymerase II transcription subunit 30
NCBI Synonym Full Names:mediator complex subunit 30
NCBI Official Symbol:MED30  
NCBI Official Synonym Symbols:MED30S; THRAP6; TRAP25  
NCBI Protein Information:mediator of RNA polymerase II transcription subunit 30
UniProt Protein Name:Mediator of RNA polymerase II transcription subunit 30
UniProt Synonym Protein Names:Mediator complex subunit 30; TRAP/Mediator complex component TRAP25; Thyroid hormone receptor-associated protein 6; Thyroid hormone receptor-associated protein complex 25 kDa component; Trap25
Protein Family:Mediator of RNA polymerase II transcription
UniProt Gene Name:MED30  
UniProt Entry Name:MED30_HUMAN
Anti-MED30 Antibody (CAB13135)Western blot analysis of extracts of Mouse brain, using MED30 Rabbit pAb (CAB13135) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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