Epigenetics & Nuclear Signaling Antibodies 2
Anti-MED25 Antibody (CAB16150)
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- SKU:
- CAB16150
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-MED25 Antibody |
| Antibody SKU: | CAB16150 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 30-200 of human MED25 (NP_112235.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | K-562, A-549, mouse brain, rat kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 30-200 of human MED25 (NP_112235.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | PYFE GLRK HYLL PAIE YFNG GPPA ETDF GGDY GGTQ YSLV VFNT VDCA PESY VQCH APTS SAYE FVTW LDGI KFMG GGGE SCSL IAEG LSTA LQLF DDFK KMRE QIGQ THRV CLLI CNSP PYLL PAVE STTY SGCT TENL VQQI GERG IHFS IVSP RKLP ALRL LFEK AAP |
| Gene ID: | 81857 |
| Uniprot: | Q71SY5 |
| Cellular Location: | Nucleus |
| Calculated MW: | 55kDa/76kDa/78kDa/82kDa/84kDa |
| Observed MW: | 78kDa |
| Synonyms: | MED25, ACID1, ARC92, BVSYS, CMT2B2, P78, PTOV2, TCBAP0758 |
| Background: | This gene encodes a component of the transcriptional coactivator complex termed the Mediator complex. This complex is required for transcription of most RNA polymerase II-dependent genes. The encoded protein plays a role in chromatin modification and in preinitiation complex assembly. Mutations in this gene are associated with Charcot-Marie-Tooth disease type 2B2. |
| UniProt Protein Function: | MED25: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for RARA/RXRA-mediated transcription. Defects in MED25 are the cause of Charcot-Marie-Tooth disease type 2B2 (CMT2B2). It is a recessive axonal form of Charcot-Marie-Tooth disease, a disorder of the peripheral nervous system, characterized by progressive weakness and atrophy, initially of the peroneal muscles and later of the distal muscles of the arms. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathies(designated CMT1 when they are dominantly inherited) and primary peripheral axonal neuropathies (CMT2). Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. Nerve conduction velocities are normal or slightly reduced. Belongs to the Mediator complex subunit 25 family. 5 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Transcription, coactivator/corepressor Chromosomal Location of Human Ortholog: 19q13.3 Cellular Component: nucleoplasm Molecular Function:retinoid X receptor binding; protein binding; retinoic acid receptor binding; transcription factor binding Biological Process: transcription initiation from RNA polymerase II promoter; negative regulation of fibroblast proliferation; gene expression; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter Disease: Basel-vanagaite-smirin-yosef Syndrome; Charcot-marie-tooth Disease, Axonal, Type 2b2 |
| NCBI Summary: | This gene encodes a component of the transcriptional coactivator complex termed the Mediator complex. This complex is required for transcription of most RNA polymerase II-dependent genes. The encoded protein plays a role in chromatin modification and in preinitiation complex assembly. Mutations in this gene are associated with Charcot-Marie-Tooth disease type 2B2. [provided by RefSeq, Apr 2010] |
| UniProt Code: | Q71SY5 |
| NCBI GenInfo Identifier: | 50428940 |
| NCBI Gene ID: | 81857 |
| NCBI Accession: | NP_112235.2 |
| UniProt Secondary Accession: | Q71SY5,O95783, Q6P143, Q6QMH5, Q707U4, Q8TB55, Q9H0L5 Q9HB34, A8K095, B9TX30, |
| UniProt Related Accession: | Q71SY5 |
| Molecular Weight: | 55,456 Da |
| NCBI Full Name: | mediator of RNA polymerase II transcription subunit 25 |
| NCBI Synonym Full Names: | mediator complex subunit 25 |
| NCBI Official Symbol: | MED25 |
| NCBI Official Synonym Symbols: | P78; ACID1; ARC92; PTOV2; CMT2B2; TCBAP0758 |
| NCBI Protein Information: | mediator of RNA polymerase II transcription subunit 25; prostate-derived protein 78 kDa; activator-recruited cofactor 92 kDa component; ARC/mediator transcriptional coactivator subunit; activator interaction domain-containing protein 1; mediator of RNA polymerase II transcription, subunit 25 homolog |
| UniProt Protein Name: | Mediator of RNA polymerase II transcription subunit 25 |
| UniProt Synonym Protein Names: | Activator interaction domain-containing protein 1; Activator-recruited cofactor 92 kDa component; ARC92; Mediator complex subunit 25; p78 |
| Protein Family: | Mediator of RNA polymerase II transcription |
| UniProt Gene Name: | MED25 |
| UniProt Entry Name: | MED25_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MED25 antibody (CAB16150) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Immunofluorescence analysis of U-2 OS cells using MED25 Polyclonal Antibody (CAB16150) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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