Name: Anti-MED24 Antibody (CAB8059)
Brand: Epigenetics & Nuclear Signaling Antibodies 4
SKU: CAB8059
Availability: OutOfStock

Antibody Name:	MED24 Rabbit Polyclonal Antibody
Antibody SKU:	CAB8059
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human MED24 (NP_055630.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	BT-474, Jurkat, Mouse brain, Mouse testis, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human MED24 (NP_055630.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MKVV NLKQ AILQ AWKE RWSD YQWA INMK KFFP KGAT WDIL NLAD ALLE QAMI GPSP NPLI LSYL KYAI SSQM VSYS SVLT AISK FDDF SRDL CVQA LLDI MDMF CDRL SCHG KAEE CIGL CRAL LSAL HWLL RCTA ASAE RLRE GLEA GTPA AGEK QLAM
Gene ID:	9862
Uniprot:	O75448
Cellular Location:	Nucleus
Calculated MW:	108kDa/110kDa
Observed MW:	110kDa

Synonyms:

MED24, ARC100, CRSP100, CRSP4, DRIP100, MED5, THRAP4, TRAP100

Background:

This gene encodes a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, possibly by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. Multiple transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	Trap100: a transcriptional coactivator protein of the thyroid hormone receptor-associated protein (TRAP) family. Forms a complex with the thyroid hormone receptor (TR) and markedly enhance TR-mediated transcription in vitro. Interacts and co-precipitates with Trap220 but does not directly bind nuclear hormone receptors.
UniProt Protein Details:	Protein type:Nuclear receptor co-regulator; Transcription, coactivator/corepressor Chromosomal Location of Human Ortholog: 17q21.1 Cellular Component: nucleoplasm; nucleus; Srb-mediator complex; ubiquitin ligase complex Molecular Function:histone acetyltransferase activity; ligand-dependent nuclear receptor transcription coactivator activity; protein binding; receptor activity; thyroid hormone receptor binding; transcription cofactor activity; vitamin D receptor binding Biological Process: androgen receptor signaling pathway; gene expression; histone acetylation; positive regulation of transcription, DNA-dependent; protein heterooligomerization; protein ubiquitination; regulation of transcription from RNA polymerase II promoter; stem cell maintenance; steroid hormone receptor signaling pathway; transcription initiation from RNA polymerase II promoter
NCBI Summary:	This gene encodes a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, possibly by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	O75448
NCBI GenInfo Identifier:	21617826
NCBI Gene ID:	9862
NCBI Accession:	O75448.1
UniProt Secondary Accession:	O75448,Q14143, Q9NNY5, A8K4S5, B3KMR9,
UniProt Related Accession:	O75448
Molecular Weight:	108,938 Da
NCBI Full Name:	Mediator of RNA polymerase II transcription subunit 24
NCBI Synonym Full Names:	mediator complex subunit 24
NCBI Official Symbol:	MED24
NCBI Official Synonym Symbols:	MED5; CRSP4; ARC100; THRAP4; CRSP100; DRIP100; TRAP100
NCBI Protein Information:	mediator of RNA polymerase II transcription subunit 24
UniProt Protein Name:	Mediator of RNA polymerase II transcription subunit 24
UniProt Synonym Protein Names:	Activator-recruited cofactor 100 kDa component; ARC100; Cofactor required for Sp1 transcriptional activation subunit 4; CRSP complex subunit 4; Mediator complex subunit 24; Thyroid hormone receptor-associated protein 4; Thyroid hormone receptor-associated protein complex 100 kDa component; Trap100; hTRAP100; Vitamin D3 receptor-interacting protein complex 100 kDa component; DRIP100
Protein Family:	Mediator of RNA polymerase II transcription
UniProt Gene Name:	MED24
UniProt Entry Name:	MED24_HUMAN

Anti-MED24 Polyclonal Antibody (CAB8059)

Western blot analysis of extracts of various cell lines, using MED24 antibody (CAB8059) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 45s.

Antibodies

MED24 Antibody (PACO01652)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)