Description
| Antibody Name: | MED24 Rabbit Polyclonal Antibody |
| Antibody SKU: | CAB8059 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human MED24 (NP_055630.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | BT-474, Jurkat, Mouse brain, Mouse testis, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human MED24 (NP_055630.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MKVV NLKQ AILQ AWKE RWSD YQWA INMK KFFP KGAT WDIL NLAD ALLE QAMI GPSP NPLI LSYL KYAI SSQM VSYS SVLT AISK FDDF SRDL CVQA LLDI MDMF CDRL SCHG KAEE CIGL CRAL LSAL HWLL RCTA ASAE RLRE GLEA GTPA AGEK QLAM |
| Gene ID: | 9862 |
| Uniprot: | O75448 |
| Cellular Location: | Nucleus |
| Calculated MW: | 108kDa/110kDa |
| Observed MW: | 110kDa |
| Synonyms: | MED24, ARC100, CRSP100, CRSP4, DRIP100, MED5, THRAP4, TRAP100 |
| Background: | This gene encodes a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, possibly by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. Multiple transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | Trap100: a transcriptional coactivator protein of the thyroid hormone receptor-associated protein (TRAP) family. Forms a complex with the thyroid hormone receptor (TR) and markedly enhance TR-mediated transcription in vitro. Interacts and co-precipitates with Trap220 but does not directly bind nuclear hormone receptors. |
| UniProt Protein Details: | Protein type:Nuclear receptor co-regulator; Transcription, coactivator/corepressor Chromosomal Location of Human Ortholog: 17q21.1 Cellular Component: nucleoplasm; nucleus; Srb-mediator complex; ubiquitin ligase complex Molecular Function:histone acetyltransferase activity; ligand-dependent nuclear receptor transcription coactivator activity; protein binding; receptor activity; thyroid hormone receptor binding; transcription cofactor activity; vitamin D receptor binding Biological Process: androgen receptor signaling pathway; gene expression; histone acetylation; positive regulation of transcription, DNA-dependent; protein heterooligomerization; protein ubiquitination; regulation of transcription from RNA polymerase II promoter; stem cell maintenance; steroid hormone receptor signaling pathway; transcription initiation from RNA polymerase II promoter |
| NCBI Summary: | This gene encodes a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, possibly by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O75448 |
| NCBI GenInfo Identifier: | 21617826 |
| NCBI Gene ID: | 9862 |
| NCBI Accession: | O75448.1 |
| UniProt Secondary Accession: | O75448,Q14143, Q9NNY5, A8K4S5, B3KMR9, |
| UniProt Related Accession: | O75448 |
| Molecular Weight: | 108,938 Da |
| NCBI Full Name: | Mediator of RNA polymerase II transcription subunit 24 |
| NCBI Synonym Full Names: | mediator complex subunit 24 |
| NCBI Official Symbol: | MED24 |
| NCBI Official Synonym Symbols: | MED5; CRSP4; ARC100; THRAP4; CRSP100; DRIP100; TRAP100 |
| NCBI Protein Information: | mediator of RNA polymerase II transcription subunit 24 |
| UniProt Protein Name: | Mediator of RNA polymerase II transcription subunit 24 |
| UniProt Synonym Protein Names: | Activator-recruited cofactor 100 kDa component; ARC100; Cofactor required for Sp1 transcriptional activation subunit 4; CRSP complex subunit 4; Mediator complex subunit 24; Thyroid hormone receptor-associated protein 4; Thyroid hormone receptor-associated protein complex 100 kDa component; Trap100; hTRAP100; Vitamin D3 receptor-interacting protein complex 100 kDa component; DRIP100 |
| Protein Family: | Mediator of RNA polymerase II transcription |
| UniProt Gene Name: | MED24 |
| UniProt Entry Name: | MED24_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MED24 antibody (CAB8059) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 45s. |
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