Name: Anti-MED23 Antibody (CAB10163)
Brand: Epigenetics & Nuclear Signaling Antibodies 1
SKU: CAB10163
Availability: OutOfStock

Antibody Name:	Anti-MED23 Antibody
Antibody SKU:	CAB10163
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 890-1110 of human MED23 (NP_001257450.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse brain, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 890-1110 of human MED23 (NP_001257450.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LLKP NDFR NRVS DFVK ENSP EHWL QNDW HTKH MNYH KKYP EKLY FEGL AEQV DPPV QIQS PYLP IYFG NVCL RFLP VFDI VIHR FLEL LPVS KSLE TLLD HLGG LYKF HDRP VTYL YNTL HYYE MHLR DRAF LKRK LVHA IIGS LKDN RPQG WCLS DTYL KCAM NARE ENPW VPDD TYYC RLIG RLVD TMAG KSPG PFPN CDWR FNEF PNPA AHAL HVTC V
Gene ID:	9439
Uniprot:	Q9ULK4
Cellular Location:	Nucleus
Calculated MW:	54kDa/100kDa/155kDa/156kDa
Observed MW:	170kDa

Synonyms:

MED23, ARC130, CRSP130, CRSP133, CRSP3, DRIP130, MRT18, SUR-2, SUR2

Background:

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. This protein also acts as a metastasis suppressor. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene.

UniProt Protein Function:	MED23: Required for transcriptional activation subsequent to the assembly of the preinitiation complex. Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for transcriptional activation by adenovirus E1A protein. Required for ELK1-dependent transcriptional activation in response to activated Ras signaling. Defects in MED23 are the cause of mental retardation autosomal recessive type 18 (MRT18). Mental retardation is characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Belongs to the Mediator complex subunit 23 family. 5 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Chromosomal Location of Human Ortholog: 6q22.33-q24.1 Cellular Component: nucleoplasm; Srb-mediator complex; transcription factor complex Molecular Function:protein binding; transcription coactivator activity Biological Process: gene expression; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; transcription initiation from RNA polymerase II promoter Disease: Mental Retardation, Autosomal Recessive 18
NCBI Summary:	The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. This protein also acts as a metastasis suppressor. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2012]
UniProt Code:	Q9ULK4
NCBI GenInfo Identifier:	28558074
NCBI Gene ID:	9439
NCBI Accession:	Q9ULK4.2
UniProt Secondary Accession:	Q9ULK4,O95403, Q5JWT3, Q5JWT4, Q6P9H6, Q9H0J2, Q9NTT9 Q9NTU0, Q9Y5P7, Q9Y667, B9TX55,
UniProt Related Accession:	Q9ULK4
Molecular Weight:	54,314 Da
NCBI Full Name:	Mediator of RNA polymerase II transcription subunit 23
NCBI Synonym Full Names:	mediator complex subunit 23
NCBI Official Symbol:	MED23
NCBI Official Synonym Symbols:	SUR2; CRSP3; MRT18; SUR-2; ARC130; CRSP130; CRSP133; DRIP130
NCBI Protein Information:	mediator of RNA polymerase II transcription subunit 23
UniProt Protein Name:	Mediator of RNA polymerase II transcription subunit 23
UniProt Synonym Protein Names:	Activator-recruited cofactor 130 kDa component; ARC130; Cofactor required for Sp1 transcriptional activation subunit 3; CRSP complex subunit 3; Mediator complex subunit 23; Protein sur-2 homolog; hSur-2; Transcriptional coactivator CRSP130; Vitamin D3 receptor-interacting protein complex 130 kDa component; DRIP130
Protein Family:	Mediator of RNA polymerase II transcription
UniProt Gene Name:	MED23
UniProt Entry Name:	MED23_HUMAN

Western blot analysis of extracts of various cell lines, using MED23 Antibody (CAB10163) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.

Antibodies

MED23 Antibody (PACO00643)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)