Name: Anti-MED21 Antibody (CAB12168)
Brand: Epigenetics & Nuclear Signaling Antibodies 1
SKU: CAB12168
Availability: OutOfStock

Antibody Name:	Anti-MED21 Antibody
Antibody SKU:	CAB12168
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-144 of human MED21 (NP_004255.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	LO2, U-87MG, 293T, A-549, 22Rv1, Mouse liver, Mouse testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-144 of human MED21 (NP_004255.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MADR LTQL QDAV NSLA DQFC NAIG VLQQ CGPP ASFN NIQT AINK DQPA NPTE EYAQ LFAA LIAR TAKD IDVL IDSL PSEE STAA LQAA SLYK LEEE NHEA ATCL EDVV YRGD MLLE KIQS ALAD IAQS QLKT RSGT HSQS LPDS
Gene ID:	9412
Uniprot:	Q13503
Cellular Location:	Nucleus
Calculated MW:	15kDa
Observed MW:	16kDa

Synonyms:	MED21, SRB7, SURB7, hSrb7
Background:	This gene encodes a member of the mediator complex subunit 21 family. The encoded protein interacts with the human RNA polymerase II holoenzyme and is involved in transcriptional regulation of RNA polymerase II transcribed genes. A pseudogene of this gene is located on chromosome 8. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	MED21: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Belongs to the Mediator complex subunit 21 family.
UniProt Protein Details:	Protein type:Nuclear receptor co-regulator; Transcription, coactivator/corepressor Chromosomal Location of Human Ortholog: 12p11.23 Cellular Component: Srb-mediator complex Molecular Function:DNA-directed RNA polymerase activity; protein binding; transcription coactivator activity Biological Process: positive regulation of transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter
NCBI Summary:	This gene encodes a member of the mediator complex subunit 21 family. The encoded protein interacts with the human RNA polymerase II holoenzyme and is involved in transcriptional regulation of RNA polymerase II transcribed genes. A pseudogene of this gene is located on chromosome 8. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012]
UniProt Code:	Q13503
NCBI GenInfo Identifier:	7531204
NCBI Gene ID:	9412
NCBI Accession:	Q13503.1
UniProt Secondary Accession:	Q13503,Q6IB05, Q92811, B2R4I3,
UniProt Related Accession:	Q13503
Molecular Weight:	16kDa
NCBI Full Name:	Mediator of RNA polymerase II transcription subunit 21
NCBI Synonym Full Names:	mediator complex subunit 21
NCBI Official Symbol:	MED21
NCBI Official Synonym Symbols:	SRB7; SURB7; hSrb7
NCBI Protein Information:	mediator of RNA polymerase II transcription subunit 21
UniProt Protein Name:	Mediator of RNA polymerase II transcription subunit 21
UniProt Synonym Protein Names:	Mediator complex subunit 21; RNA polymerase II holoenzyme component SRB7; RNAPII complex component SRB7; hSrb7
Protein Family:	Putative mediator of RNA polymerase II transcription
UniProt Gene Name:	MED21

Western blot analysis of extracts of various cell lines, using MED21 antibody (CAB12168) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.

Antibodies

MED21 Antibody (PACO05756)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)