Name: Anti-MED20 Antibody (CAB15757)
Brand: Epigenetics & Nuclear Signaling Antibodies 2
SKU: CAB15757
Availability: OutOfStock

Antibody Name:	Anti-MED20 Antibody
Antibody SKU:	CAB15757
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-212 of human MED20 (NP_004266.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	DU145, Mouse brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-212 of human MED20 (NP_004266.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MGVT CVSQ MPVA EGKS VQQT VELL TRKL EMLG AEKQ GTFC VDCE TYHT AAST LGSQ GQTG KLMY VMHN SEYP LSCF ALFE NGPC LIAD TNFD VLMV KLKG FFQS AKAS KIET RGTR YQYC DFLV KVGT VTMG PSAR GISV EVEY GPCV VASD CWSL LLEF LQSF LGSH TPGA PAVF GNRH DAVY GPAD TMVQ YMEL FNKI RKQQ QVPV AGIR
Gene ID:	9477
Uniprot:	Q9H944
Cellular Location:	Nucleus
Calculated MW:	13kDa/23kDa
Observed MW:	24kDa

Synonyms:

MED20, PRO0213, TRFP, SRB2

Background:

This gene encodes a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. A mutation in this gene has been associated with a novel infantile-onset neurodegenerative movement disorder. Alternatively spliced transcript variants have been identified.

UniProt Protein Function:	TRFP: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Belongs to the Mediator complex subunit 20 family.
UniProt Protein Details:	Chromosomal Location of Human Ortholog: 6p21.1 Cellular Component: nucleoplasm; Srb-mediator complex Molecular Function:protein binding; DNA-directed RNA polymerase activity Biological Process: regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; transcription, DNA-dependent; gene expression
NCBI Summary:	MED20 is a subunit of the Mediator complex, a multiprotein coactivator of RNA transcription that interacts with DNA-bound transcriptional activators, RNA polymerase II (see MIM 180660), and general initiation factors (Sato et al., 2003 [PubMed 14576168]).[supplied by OMIM, Aug 2009]
UniProt Code:	Q9H944
NCBI GenInfo Identifier:	30023851
NCBI Gene ID:	9477
NCBI Accession:	NP_004266.2
UniProt Secondary Accession:	Q9H944,O95821, Q5T8J4, Q9Y429, B4DE08,
UniProt Related Accession:	Q9H944
Molecular Weight:	13,540 Da
NCBI Full Name:	mediator of RNA polymerase II transcription subunit 20
NCBI Synonym Full Names:	mediator complex subunit 20
NCBI Official Symbol:	MED20
NCBI Official Synonym Symbols:	TRFP; PRO0213
NCBI Protein Information:	mediator of RNA polymerase II transcription subunit 20; TRF-proximal protein homolog; Trf (TATA binding protein-related factor)-proximal homolog; hTRFP; mediator complex subunit 20mediator complex subunit 20mediator complex subunit 20Trf (TATA binding protein-related factor)-proximal homolog
UniProt Protein Name:	Mediator of RNA polymerase II transcription subunit 20
UniProt Synonym Protein Names:	Mediator complex subunit 20; TRF-proximal protein homolog; hTRFP
Protein Family:	Putative mediator of RNA polymerase II transcription
UniProt Gene Name:	MED20
UniProt Entry Name:	MED20_HUMAN

Western blot analysis of extracts of various cell lines, using MED20 antibody (CAB15757) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Antibodies

MED20 Antibody (PACO10442)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)