Epigenetics & Nuclear Signaling Antibodies 3
Anti-MED18 Antibody (CAB14588)
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- SKU:
- CAB14588
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-MED18 Antibody |
| Antibody SKU: | CAB14588 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 30-110 of human MED18 (NP_060108.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | HeLa |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 30-110 of human MED18 (NP_060108.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | HSLE SLIH RLRG LCDN MEPE TFLD HEMV FLLK GQQA SPFV LRAR RSMD RAGA PWHL RYLG QPEM GDKN RHAL VRNC VDIA T |
| Gene ID: | 54797 |
| Uniprot: | Q9BUE0 |
| Cellular Location: | Nucleus |
| Calculated MW: | 23kDa |
| Observed MW: | 24kDa |
| Synonyms: | MED18, p28b, SRB5 |
| Background: |
| UniProt Protein Function: | MED18: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Belongs to the Mediator complex subunit 18 family. |
| UniProt Protein Details: | Protein type:Nuclear receptor co-regulator Chromosomal Location of Human Ortholog: 1p35.3 Cellular Component: Srb-mediator complex Molecular Function:protein binding Biological Process: termination of RNA polymerase II transcription |
| NCBI Summary: | MED18 is a component of the Mediator complex, which is a coactivator for DNA-binding factors that activate transcription via RNA polymerase II (Sato et al., 2003 [PubMed 12584197]).[supplied by OMIM, Oct 2008] |
| UniProt Code: | Q9BUE0 |
| NCBI GenInfo Identifier: | 74752353 |
| NCBI Gene ID: | 54797 |
| NCBI Accession: | Q9BUE0.1 |
| UniProt Secondary Accession: | Q9BUE0,Q9NXU9, D3DPM1, |
| UniProt Related Accession: | Q9BUE0 |
| Molecular Weight: | 23,663 Da |
| NCBI Full Name: | Mediator of RNA polymerase II transcription subunit 18 |
| NCBI Synonym Full Names: | mediator complex subunit 18 |
| NCBI Official Symbol: | MED18 |
| NCBI Official Synonym Symbols: | p28b |
| NCBI Protein Information: | mediator of RNA polymerase II transcription subunit 18 |
| UniProt Protein Name: | Mediator of RNA polymerase II transcription subunit 18 |
| UniProt Synonym Protein Names: | Mediator complex subunit 18; p28b |
| Protein Family: | Mediator of RNA polymerase II transcription |
| UniProt Gene Name: | MED18 |
| UniProt Entry Name: | MED18_HUMAN |
 | Western blot analysis of extracts of HeLa cells, using MED18 antibody (CAB14588) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s. |
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