Name: Anti-MED17 Antibody (CAB8716)
Brand: Epigenetics & Nuclear Signaling Antibodies 4
SKU: CAB8716
Availability: OutOfStock

Antibody Name:	Anti-MED17 Antibody
Antibody SKU:	CAB8716
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human MED17 (NP_004259.3).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	LO2, 293T, HeLa

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human MED17 (NP_004259.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSGV RAVR ISIE SACE KQVH EVGL DGTE TYLP PLSM SQNL ARLA QRID FSQG SGSE EEEA AGTE GDAQ EWPG AGSS ADQD DEEG VVKF QPSL WPWD SVRN NLRS ALTE MCVL YDVL SIVR DKKF MTLD PVSQ DALP PKQN PQTL QLIS KKKS LAGA AQIL LKGA ERLT KSVT ENQE NKLQ RDFN SELL RLRQ HWKL RKVG DKIL GDLS YRSA GSLF PHHG TFEV IKNT DLDL DKKI PEDY CPLD VQIP SDLE GSAY IKVS IQKQ APDI GDLG TVNL FKRP LPKS KPGS PHWQ TKLE AAQN
Gene ID:	9440
Uniprot:	Q9NVC6
Cellular Location:	Nucleus
Calculated MW:	15kDa/72kDa
Observed MW:	80kDa

Synonyms:

MED17, CRSP6, CRSP77, DRIP80, TRAP80, SRB4

Background:

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors.

UniProt Protein Function:	MED17: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Defects in MED17 are the cause of microcephaly postnatal progressive with seizures and brain atrophy (MCPHSBA). It is a disorder characterized by postnatal progressive microcephaly and severe developmental retardation associated with cerebral and cerebellar atrophy. Infants manifest swallowing difficulties leading to failure to thrive, jitteriness, poor visual fixation, truncal arching, seizures. There is no acquisition of developmental milestones and patients suffer from marked spasticity and profound retardation. Progressive microcephaly becomes evident few months after birth. Belongs to the Mediator complex subunit 17 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Nuclear receptor co-regulator; Transcription, coactivator/corepressor Chromosomal Location of Human Ortholog: 11q14 Cellular Component: membrane; nucleoplasm; nucleus; Srb-mediator complex; transcription factor complex Molecular Function:protein binding; receptor activity; thyroid hormone receptor binding; transcription coactivator activity; transcription cofactor activity Biological Process: androgen receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of transcription from RNA polymerase II promoter; steroid hormone receptor signaling pathway; transcription initiation from RNA polymerase II promoter Disease: Microcephaly, Postnatal Progressive, With Seizures And Brain Atrophy
NCBI Summary:	The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9NVC6
NCBI GenInfo Identifier:	296437366
NCBI Gene ID:	9440
NCBI Accession:	Q9NVC6.2
UniProt Secondary Accession:	Q9NVC6,Q9HA81, Q9UNP7, Q9Y2W0, Q9Y660, B3KN07,
UniProt Related Accession:	Q9NVC6
Molecular Weight:	15,951 Da
NCBI Full Name:	Mediator of RNA polymerase II transcription subunit 17
NCBI Synonym Full Names:	mediator complex subunit 17
NCBI Official Symbol:	MED17
NCBI Official Synonym Symbols:	CRSP6; CRSP77; DRIP80; TRAP80
NCBI Protein Information:	mediator of RNA polymerase II transcription subunit 17
UniProt Protein Name:	Mediator of RNA polymerase II transcription subunit 17
UniProt Synonym Protein Names:	Activator-recruited cofactor 77 kDa component; ARC77; Cofactor required for Sp1 transcriptional activation subunit 6; CRSP complex subunit 6; Mediator complex subunit 17; Thyroid hormone receptor-associated protein complex 80 kDa component; Trap80; Transcriptional coactivator CRSP77; Vitamin D3 receptor-interacting protein complex 80 kDa component; DRIP80
Protein Family:	Mediator of RNA polymerase II transcription
UniProt Gene Name:	MED17
UniProt Entry Name:	MED17_HUMAN

Anti-MED17 Polyclonal Antibody (CAB8716)

Western blot analysis of extracts of various cell lines, using MED17 antibody (CAB8716) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Antibodies

MED17 Antibody (PACO00644)

MED17 Antibody (PACO10440)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)