Epigenetics & Nuclear Signaling Antibodies 1
Anti-MED14 Antibody (CAB10455)
- SKU:
- CAB10455
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
Antibody Name: | Anti-MED14 Antibody |
Antibody SKU: | CAB10455 |
Antibody Size: | 20uL, 50uL, 100uL |
Application: | WB |
Reactivity: | Human, Mouse, Rat |
Host Species: | Rabbit |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 470-650 of human MED14 (NP_004220.2). |
Application: | WB |
Recommended Dilution: | WB 1:500 - 1:2000 |
Reactivity: | Human, Mouse, Rat |
Positive Samples: | HeLa, SW480, Jurkat, Mouse brain, Mouse pancreas |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 470-650 of human MED14 (NP_004220.2). |
Purification Method: | Affinity purification |
Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Isotype: | IgG |
Sequence: | LDQA TLDD MEKS VNDD MKRI IPWI QQLK FWLG QQRC KQSI KHLP TISS ETLQ LSNY STHP IGNL SKNK LFIK LTRL PQYY IVVE MLEV PNKP TQLS YKYY FMSV NAAD REDS PAMA LLLQ QFKE NIQD LVFR TKTG KQTR TNAK RKLS DDPC PVES KKTK RAGE MCAF NKVL AHFV AMCD T |
Gene ID: | 9282 |
Uniprot: | O60244 |
Cellular Location: | Nucleus |
Calculated MW: | 160kDa |
Observed MW: | 150kDa |
Synonyms: | MED14, CRSP150, CRSP2, CSRP, CXorf4, DRIP150, EXLM1, RGR1, TRAP170 |
Background: | The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. This protein contains a bipartite nuclear localization signal. This gene is known to escape chromosome X-inactivation. |
UniProt Protein Function: | Trap170: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Belongs to the Mediator complex subunit 14 family. |
UniProt Protein Details: | Protein type:Nuclear receptor co-regulator; Transcription, coactivator/corepressor Chromosomal Location of Human Ortholog: Xp11.4 Cellular Component: membrane; nucleoplasm; nucleus; Srb-mediator complex Molecular Function:protein binding; receptor activity; transcription coactivator activity; transcription cofactor activity Biological Process: androgen receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of transcription from RNA polymerase II promoter; steroid hormone receptor signaling pathway; transcription initiation from RNA polymerase II promoter |
NCBI Summary: | The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. This protein contains a bipartite nuclear localization signal. This gene is known to escape chromosome X-inactivation. [provided by RefSeq, Jul 2008] |
UniProt Code: | O60244 |
NCBI GenInfo Identifier: | 145559461 |
NCBI Gene ID: | 9282 |
NCBI Accession: | O60244.2 |
UniProt Secondary Accession: | O60244,Q4KMR7, Q9UNB3, |
UniProt Related Accession: | O60244 |
Molecular Weight: | 160,607 Da |
NCBI Full Name: | Mediator of RNA polymerase II transcription subunit 14 |
NCBI Synonym Full Names: | mediator complex subunit 14 |
NCBI Official Symbol: | MED14 |
NCBI Official Synonym Symbols: | CSRP; RGR1; CRSP2; EXLM1; CXorf4; CRSP150; DRIP150; TRAP170 |
NCBI Protein Information: | mediator of RNA polymerase II transcription subunit 14 |
UniProt Protein Name: | Mediator of RNA polymerase II transcription subunit 14 |
UniProt Synonym Protein Names: | Activator-recruited cofactor 150 kDa component; ARC150; Cofactor required for Sp1 transcriptional activation subunit 2; CRSP complex subunit 2; Mediator complex subunit 14; RGR1 homolog; hRGR1; Thyroid hormone receptor-associated protein complex 170 kDa component; Trap170; Transcriptional coactivator CRSP150; Vitamin D3 receptor-interacting protein complex 150 kDa component; DRIP150 |
Protein Family: | Mediator of RNA polymerase II transcription |
UniProt Gene Name: | MED14 |
UniProt Entry Name: | MED14_HUMAN |
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