Antibody Name:	Anti-MECP2 Antibody [KO Validated]
Antibody SKU:	CAB0707
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human MECP2 (NP_004983.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:1000 IHC 1:50 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	MCF7, SW620, Jurkat, Mouse lung, Mouse brain, 293T

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human MECP2 (NP_004983.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MVAG MLGL REEK SEDQ DLQG LKDK PLKF KKVK KDKK EEKE GKHE PVQP SAHH SAEP AEAG KAET SEGS GSAP AVPE ASAS PKQR RSII RDRG PMYD DPTL PEGW TRKL KQRK SGRS AGKY DVYL INPQ GKAF RSKV ELIA YFEK VGDT SLDP NDFD FTVT GRGS PSRR EQKP PKKP KSPK APGT GRGR GRPK GSGT TRPK AATS EGVQ VKRV LEKS PGKL LVKM PFQT SPGG KAEG GGAT TSTQ VMVI KRPG RKRK AEAD PQAI PKKR GRKP GSVV AAAA
Gene ID:	4204
Uniprot:	P51608
Cellular Location:	Nucleus
Calculated MW:	52kDa/53kDa
Observed MW:	60kDa/80kDa

Synonyms:

MECP2, AUTSX3, MRX16, MRX79, MRXS13, MRXSL, PPMX, RS, RTS, RTT

Background:

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of most cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females. Alternative splicing results in multiple transcript variants encoding different isoforms.

UniProt Protein Function:	Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5-methylcytosine (5mC).
NCBI Summary:	DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of most cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2015]
UniProt Code:	P51608
NCBI GenInfo Identifier:	1708973
NCBI Gene ID:	4204
NCBI Accession:	P51608.1
UniProt Secondary Accession:	P51608,O15233, Q6QHH9, Q7Z384,
UniProt Related Accession:	P51608
Molecular Weight:	53,323 Da
NCBI Full Name:	Methyl-CpG-binding protein 2
NCBI Synonym Full Names:	methyl-CpG binding protein 2
NCBI Official Symbol:	MECP2
NCBI Official Synonym Symbols:	RS; RTS; RTT; PPMX; MRX16; MRX79; MRXSL; AUTSX3; MRXS13
NCBI Protein Information:	methyl-CpG-binding protein 2
UniProt Protein Name:	Methyl-CpG-binding protein 2
Protein Family:	Methyl-CpG-binding protein
UniProt Gene Name:	MECP2
UniProt Entry Name:	MECP2_HUMAN

	Western blot analysis of extracts of various cell lines, using MECP2 antibody (CAB0707) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Western blot analysis of extracts from normal (control) and MECP2 Rabbit pAb knockout (KO) 293T cells, using MECP2 Rabbit pAb antibody (CAB0707) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded human gastric cancer using MECP2 antibody (CAB0707) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using MECP2 antibody (CAB0707) at dilution of 1:100 (40x lens).