Antibody Name:	Anti-ME2 Antibody
Antibody SKU:	CAB9650
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IP
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 220-479 of human ME2 (NP_001161807.1).

Application:	WB IP
Recommended Dilution:	WB 1:500 - 1:2000 IP 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	LO2, HL-60, SKOV3, HT-1080

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 220-479 of human ME2 (NP_001161807.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	GLYQ KRDR TQQY DDLI DEFM KAIT DRYG RNTL IQFE DFGN HNAF RFLR KYRE KYCT FNDD IQGT AAVA LAGL LAAQ KVIS KPIS EHKI LFLG AGEA ALGI ANLI VMSM VENG LSEQ EAQK KIWM FDKY GLLV KGRK AKID SYQE PFTH SAPE SIPD TFED AVNI LKPS TIIG VAGA GRLF TPDV IRAM ASIN ERPV IFAL SNPT AQAE CTAE EAYT LTEG RCLF ASGS PFGP VKLT DGRV FTPG QGNN VYIF PGYR IPIC
Gene ID:	4200
Uniprot:	P23368
Cellular Location:	Mitochondrion matrix
Calculated MW:	53kDa/65kDa
Observed MW:	59kDa/65kDa

Synonyms:

ME2, ODS1

Background:

This gene encodes a mitochondrial NAD-dependent malic enzyme, a homotetrameric protein, that catalyzes the oxidative decarboxylation of malate to pyruvate. It had previously been weakly linked to a syndrome known as Friedreich ataxia that has since been shown to be the result of mutation in a completely different gene. Certain single-nucleotide polymorphism haplotypes of this gene have been shown to increase the risk for idiopathic generalized epilepsy. Alternatively spliced transcript variants encoding different isoforms found for this gene.

UniProt Protein Function:	ME2: a mitochondrial NAD-dependent malic enzyme, a homotetrameric protein, that catalyzes the oxidative decarboxylation of malate to pyruvate. It had previously been weakly linked to a syndrome known as Friedreich ataxia that has since been shown to be the result of mutation in a completely different gene. Certain single-nucleotide polymorphism haplotypes of this gene have been shown to increase the risk for idiopathic generalized epilepsy. Alternatively spliced transcript variants encoding different isoforms found for this gene. [provided by RefSeq, Dec 2009]
UniProt Protein Details:	Protein type:Carbohydrate Metabolism - pyruvate; Oxidoreductase; Mitochondrial; EC 1.1.1.38 Chromosomal Location of Human Ortholog: 18q21 Cellular Component: intracellular membrane-bound organelle; mitochondrion Molecular Function:electron carrier activity; malic enzyme activity Disease: Epilepsy, Idiopathic Generalized; Opioid Dependence, Susceptibility To, 1
NCBI Summary:	This gene encodes a mitochondrial NAD-dependent malic enzyme, a homotetrameric protein, that catalyzes the oxidative decarboxylation of malate to pyruvate. It had previously been weakly linked to a syndrome known as Friedreich ataxia that has since been shown to be the result of mutation in a completely different gene. Certain single-nucleotide polymorphism haplotypes of this gene have been shown to increase the risk for idiopathic generalized epilepsy. Alternatively spliced transcript variants encoding different isoforms found for this gene. [provided by RefSeq, Dec 2009]
UniProt Code:	P23368
NCBI GenInfo Identifier:	126733
NCBI Gene ID:	4200
NCBI Accession:	P23368.1
UniProt Secondary Accession:	P23368,Q9BWL6, Q9BYG1, Q9H4B2, B2R8J2,
UniProt Related Accession:	P23368
Molecular Weight:	53,586 Da
NCBI Full Name:	NAD-dependent malic enzyme, mitochondrial
NCBI Synonym Full Names:	malic enzyme 2
NCBI Official Symbol:	ME2
NCBI Official Synonym Symbols:	ODS1
NCBI Protein Information:	NAD-dependent malic enzyme, mitochondrial
UniProt Protein Name:	NAD-dependent malic enzyme, mitochondrial
UniProt Synonym Protein Names:	Malic enzyme 2
UniProt Gene Name:	ME2
UniProt Entry Name:	MAOM_HUMAN

	Western blot analysis of extracts of various cell lines, using ME2 antibody (CAB9650) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunoprecipitation analysis of 200ug extracts of SKOV3 cells using 3ug ME2 antibody (CAB9650). Western blot was performed from the immunoprecipitate using ME2 antibody (CAB9650) at a dilition of 1:1000.