Cell Biology Antibodies 7
Anti-MDM4 Antibody (CAB1747)
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- SKU:
- CAB1747
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-MDM4 Antibody |
| Antibody SKU: | CAB1747 |
| Antibody Size: | 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide of human MDM4 |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:1000 |
| Reactivity: | Human |
| Positive Samples: | ZR-75-1 |
| Immunogen: | A synthetic peptide of human MDM4 |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 4194 |
| Uniprot: | O15151 |
| Cellular Location: | Nucleus |
| Calculated MW: | 13kDa/14kDa/18kDa/49kDa/54kDa |
| Observed MW: | 47kDa |
| Synonyms: | MDM4, HDMX, MDMX, MRP1 |
| Background: | This gene encodes a nuclear protein that contains a p53 binding domain at the N-terminus and a RING finger domain at the C-terminus, and shows structural similarity to p53-binding protein MDM2. Both proteins bind the p53 tumor suppressor protein and inhibit its activity, and have been shown to be overexpressed in a variety of human cancers. However, unlike MDM2 which degrades p53, this protein inhibits p53 by binding its transcriptional activation domain. This protein also interacts with MDM2 protein via the RING finger domain, and inhibits the latter's degradation. So this protein can reverse MDM2-targeted degradation of p53, while maintaining suppression of p53 transactivation and apoptotic functions. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. |
| UniProt Protein Function: | MDM4: Inhibits p53/TP53- and TP73/p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Inhibits degradation of MDM2. Can reverse MDM2-targeted degradation of TP53 while maintaining suppression of TP53 transactivation and apoptotic functions. Interacts with MDM2, TP53, TP73 and USP2. Found in a trimeric complex with UPB2, MDM2 and MDM4. Interacts (phosphorylated) with YWHAG; negatively regulates MDM4 activity toward TP53. Down-regulated by cisplatin. Expressed in all tissues tested with high levels in thymus. Belongs to the MDM2/MDM4 family. 5 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Ubiquitin ligase; Ligase; Ubiquitin conjugating system; EC 6.3.2.- Chromosomal Location of Human Ortholog: 1q32 Cellular Component: nucleoplasm; nucleus Molecular Function:enzyme binding; protein binding; zinc ion binding Biological Process: cell proliferation; DNA damage response, signal transduction by p53 class mediator; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; G0 to G1 transition; negative regulation of cell proliferation; negative regulation of protein catabolic process; negative regulation of transcription from RNA polymerase II promoter; protein complex assembly; protein stabilization |
| NCBI Summary: | This gene encodes a nuclear protein that contains a p53 binding domain at the N-terminus and a RING finger domain at the C-terminus, and shows structural similarity to p53-binding protein MDM2. Both proteins bind the p53 tumor suppressor protein and inhibit its activity, and have been shown to be overexpressed in a variety of human cancers. However, unlike MDM2 which degrades p53, this protein inhibits p53 by binding its transcriptional activation domain. This protein also interacts with MDM2 protein via the RING finger domain, and inhibits the latter's degradation. So this protein can reverse MDM2-targeted degradation of p53, while maintaining suppression of p53 transactivation and apoptotic functions. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Feb 2011] |
| UniProt Code: | O15151 |
| NCBI GenInfo Identifier: | 76803799 |
| NCBI Gene ID: | 4194 |
| NCBI Accession: | O15151.2 |
| UniProt Secondary Accession: | O15151,Q2M2Y2, Q32SL2, Q6GS18, Q8IV83, |
| UniProt Related Accession: | O15151 |
| Molecular Weight: | 49,541 Da |
| NCBI Full Name: | Protein Mdm4 |
| NCBI Synonym Full Names: | MDM4, p53 regulator |
| NCBI Official Symbol: | MDM4 |
| NCBI Official Synonym Symbols: | HDMX; MDMX; MRP1 |
| NCBI Protein Information: | protein Mdm4 |
| UniProt Protein Name: | Protein Mdm4 |
| UniProt Synonym Protein Names: | Double minute 4 protein; Mdm2-like p53-binding protein; Protein Mdmx; p53-binding protein Mdm4 |
| Protein Family: | Protein |
| UniProt Gene Name: | MDM4 |
| UniProt Entry Name: | MDM4_HUMAN |
 | Western blot analysis of extracts of ZR-75-1 cells, using MDM4 antibody (CAB1747). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
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