Metabolism Antibodies 1

Anti-MDH2 Antibody (CAB13516)

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SKU:
CAB13516
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-MDH2 Antibody
Antibody SKU:CAB13516
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 59-338 of human MDH2 (NP_005909.2).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:293T, HepG2, Jurkat, A-549, MCF7, HeLa, Mouse kidney, Mouse heart, Mouse skeletal muscle
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 59-338 of human MDH2 (NP_005909.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:AHTP GVAA DLSH IETK AAVK GYLG PEQL PDCL KGCD VVVI PAGV PRKP GMTR DDLF NTNA TIVA TLTA ACAQ HCPE AMIC VIAN PVNS TIPI TAEV FKKH GVYN PNKI FGVT TLDI VRAN TFVA ELKG LDPA RVNV PVIG GHAG KTII PLIS QCTP KVDF PQDQ LTAL TGRI QEAG TEVV KAKA GAGS ATLS MAYA GARF VFSL VDAM NGKE GVVE CSFV KSQE TECT YFST PLLL GKKG IEKN LGIG KVSS FEEK MISD AIPE LKAS IKKG EDFV KTLK
Gene ID:4191
Uniprot:P40926
Cellular Location:Mitochondrion matrix
Calculated MW:30kDa/35kDa
Observed MW:35kDa
Synonyms:MDH2, EIEE51, M-MDH, MDH, MGC:3559, MOR1
Background:Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the mitochondria and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. Several transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:MDH2: mitochondrial malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. May play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria.
UniProt Protein Details:

Protein type:Carbohydrate Metabolism - pyruvate; Carbohydrate Metabolism - citrate (TCA) cycle; Mitochondrial; Carbohydrate Metabolism - glyoxylate and dicarboxylate; EC 1.1.1.37; Oxidoreductase

Chromosomal Location of Human Ortholog: 7cen-q22

Cellular Component: nucleoplasm; mitochondrion; mitochondrial matrix; mitochondrial inner membrane; plasma membrane; nucleus

Molecular Function:protein self-association; L-malate dehydrogenase activity; malate dehydrogenase (NADP+) activity

Biological Process: cellular metabolic process; malate metabolic process; oxaloacetate metabolic process; NADH metabolic process; tricarboxylic acid cycle; carbohydrate metabolic process; glucose metabolic process; pathogenesis; cellular carbohydrate metabolic process; internal protein amino acid acetylation; gluconeogenesis

NCBI Summary:Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the mitochondria and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2013]
UniProt Code:P40926
NCBI GenInfo Identifier:215274114
NCBI Gene ID:4191
NCBI Accession:P40926.3
UniProt Secondary Accession:P40926,O43682, A8K414, B2RE78, B4DE44, E9PDB2,
UniProt Related Accession:P40926
Molecular Weight:338
NCBI Full Name:Malate dehydrogenase, mitochondrial
NCBI Synonym Full Names:malate dehydrogenase 2, NAD (mitochondrial)
NCBI Official Symbol:MDH2  
NCBI Official Synonym Symbols:MDH; MOR1; M-MDH; MGC:3559  
NCBI Protein Information:malate dehydrogenase, mitochondrial
UniProt Protein Name:Malate dehydrogenase, mitochondrial
Protein Family:Malate dehydrogenase
UniProt Gene Name:MDH2  
UniProt Entry Name:MDHM_HUMAN
Anti-MDH2 Antibody (CAB13516)Western blot analysis of extracts of various cell lines, using MDH2 antibody (CAB13516) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-MDH2 Antibody (CAB13516)Immunofluorescence analysis of U2OS cells using MDH2 antibody (CAB13516). Blue: DAPI for nuclear staining.
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