Metabolism Antibodies 2
Anti-MDH1 Antibody (CAB7563)
- SKU:
- CAB7563
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-MDH1 Antibody |
| Antibody SKU: | CAB7563 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-334 of human MDH1 (NP_005908.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HepG2, THP-1, HeLa, Jurkat, Mouse liver, Mouse kidney, Mouse heart, Mouse eye, Rat kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-334 of human MDH1 (NP_005908.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MSEP IRVL VTGA AGQI AYSL LYSI GNGS VFGK DQPI ILVL LDIT PMMG VLDG VLME LQDC ALPL LKDV IATD KEDV AFKD LDVA ILVG SMPR REGM ERKD LLKA NVKI FKSQ GAAL DKYA KKSV KVIV VGNP ANTN CLTA SKSA PSIP KENF SCLT RLDH NRAK AQIA LKLG VTAN DVKN VIIW GNHS STQY PDVN HAKV KLQG KEVG VYEA LKDD SWLK GEFV TTVQ QRGA AVIK ARKL SSAM SAAK AICD HVRD IWFG TPEG EFVS MGVI SDGN SYGV PDDL LYSF PVVI KNKT WKFV EGLP INDF SREK MDLT AKEL TEEK ESAF EFLS SA |
| Gene ID: | 4190 |
| Uniprot: | P40925 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 27kDa/36kDa/38kDa |
| Observed MW: | 36kDa |
| Synonyms: | MDH1, HEL-S-32, MDH-s, MDHA, MGC:1375, MOR2 |
| Background: | This gene encodes an enzyme that catalyzes the NAD/NADH-dependent, reversible oxidation of malate to oxaloacetate in many metabolic pathways, including the citric acid cycle. Two main isozymes are known to exist in eukaryotic cells: one is found in the mitochondrial matrix and the other in the cytoplasm. This gene encodes the cytosolic isozyme, which plays a key role in the malate-aspartate shuttle that allows malate to pass through the mitochondrial membrane to be transformed into oxaloacetate for further cellular processes. Alternatively spliced transcript variants have been found for this gene. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Pseudogenes have been identified on chromosomes X and 6. |
| UniProt Protein Function: | MDH1: Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the cytoplasm and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.[provided by RefSeq, Nov 2010] |
| UniProt Protein Details: | Protein type:EC 1.1.1.96; Carbohydrate Metabolism - pyruvate; Oxidoreductase; Carbohydrate Metabolism - citrate (TCA) cycle; EC 1.1.1.37; Carbohydrate Metabolism - glyoxylate and dicarboxylate Chromosomal Location of Human Ortholog: 2p13.3 Cellular Component: centrosome; extracellular space; mitochondrion; cytoplasm; cytosol Molecular Function:malic enzyme activity; L-malate dehydrogenase activity; diiodophenylpyruvate reductase activity; NAD binding Biological Process: malate metabolic process; oxaloacetate metabolic process; NADH metabolic process; tricarboxylic acid cycle; carbohydrate metabolic process; glucose metabolic process; cellular carbohydrate metabolic process; pathogenesis; gluconeogenesis |
| NCBI Summary: | This gene encodes an enzyme that catalyzes the NAD/NADH-dependent, reversible oxidation of malate to oxaloacetate in many metabolic pathways, including the citric acid cycle. Two main isozymes are known to exist in eukaryotic cells: one is found in the mitochondrial matrix and the other in the cytoplasm. This gene encodes the cytosolic isozyme, which plays a key role in the malate-aspartate shuttle that allows malate to pass through the mitochondrial membrane to be transformed into oxaloacetate for further cellular processes. Alternatively spliced transcript variants have been found for this gene. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Pseudogenes have been identified on chromosomes X and 6. [provided by RefSeq, Feb 2016] |
| UniProt Code: | P40925 |
| NCBI GenInfo Identifier: | 1708967 |
| NCBI Gene ID: | 4190 |
| NCBI Accession: | P40925.4 |
| UniProt Related Accession: | P40925 |
| Molecular Weight: | ~ 36kDa |
| NCBI Full Name: | Malate dehydrogenase, cytoplasmic |
| NCBI Synonym Full Names: | malate dehydrogenase 1 |
| NCBI Official Symbol: | MDH1 |
| NCBI Official Synonym Symbols: | MDHA; MOR2; MDH-s; HEL-S-32; MGC:1375 |
| NCBI Protein Information: | malate dehydrogenase, cytoplasmic; malate dehydrogenase, peroxisomal |
| UniProt Protein Name: | Malate dehydrogenase, cytoplasmic |
| UniProt Synonym Protein Names: | Cytosolic malate dehydrogenase; Diiodophenylpyruvate reductase (EC:1.1.1.96) |
| Protein Family: | Malate dehydrogenase |
| UniProt Gene Name: | MDH1 |
| UniProt Entry Name: | MDHC_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MDH1 antibody (CAB7563) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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