Name: Anti-MCPH1 Antibody (CAB5472)
Brand: Cell Biology Antibodies 9
SKU: CAB5472
Availability: OutOfStock

Antibody Name:	Anti-MCPH1 Antibody
Antibody SKU:	CAB5472
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 263-562 of human MCPH1 (NP_001166046.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	A-549, HeLa, 293T, 3T3L1, SH-SY5Y, Mouse testis, Mouse brain, Mouse liver, Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 263-562 of human MCPH1 (NP_001166046.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	VTPD QKQA AGMS QETF EEKY RLSP TLSS TKGH LLIH SRPR SSSV KRKR VSHG SHSP PKEK CKRK RSTR RSIM PRLQ LCRS EDRL QHVA GPAL EALS CGES SYDD YFSP DNLK ERYS ENLP PESQ LPSS PAQL SCRS LSKK ERTS IFEM SDFS CVGK KTRT VDIT NFTA KTIS SPRK TGNG EGRA TSSC VTSA PEEA LRCC RQAG KEDA CPEG NGFS YTIE DPAL PKGH DDDL TPLE GSLE EMKE AVGL KSTQ NKGT TSKI SNSS EGEA QSEH EPCF IVDC NMET STEE KENL PGGY SGSM
Gene ID:	79648
Uniprot:	Q8NEM0
Cellular Location:	Cytoplasm, centrosome, cytoskeleton, microtubule organizing center
Calculated MW:	62kDa/67kDa/92kDa
Observed MW:	60kDa

Synonyms:	MCPH1, BRIT1, MCT
Background:	This gene encodes a DNA damage response protein. The encoded protein may play a role in G2/M checkpoint arrest via maintenance of inhibitory phosphorylation of cyclin-dependent kinase 1. Mutations in this gene have been associated with primary autosomal recessive microcephaly 1 and premature chromosome condensation syndrome. Alternatively spliced transcript variants have been described.

UniProt Protein Function:	MCPH1: a DNA damage responsive protein and transcriptional repressor of human telomere reverse transcriptase. An early responder to DNA damage. Required for double strand break repair via homologous recombination and nonhomologous end joining. Promotes chromatin relaxation by recruiting and maintaining the chromatin remodeling SWI-SNF complex at sites of DNA double strand breakage. Participates in the ATR/Chk1-mediated DNA checkpoint in humans and transcriptionally regulates BRCA1 and Chk1. Plays a role in neurogenesis and regulation of the size of the cerebral cortex. Mutations of MCPH1 can cause microcephaly in humans and appears to play an evolutionarily conserved role in brain development. Alterations have been identified in breast, ovarian and prostate cancer. Appears to coordinate S-M transitions in fly embryos.
UniProt Protein Details:	Protein type:Cell cycle regulation Chromosomal Location of Human Ortholog: 8p23.1 Cellular Component: nucleoplasm; cytoplasm; microtubule organizing center Molecular Function:identical protein binding; protein binding Biological Process: cerebral cortex development; mitotic cell cycle Disease: Microcephaly 1, Primary, Autosomal Recessive
NCBI Summary:	This gene encodes a DNA damage response protein. The encoded protein may play a role in G2/M checkpoint arrest via maintenance of inhibitory phosphorylation of cyclin-dependent kinase 1. Mutations in this gene have been associated with primary autosomal recessive microcephaly 1 and premature chromosome condensation syndrome. Alternatively spliced transcript variants have been described. [provided by RefSeq, Feb 2010]
UniProt Code:	Q8NEM0
NCBI GenInfo Identifier:	296439305
NCBI Gene ID:	79648
NCBI Accession:	Q8NEM0.3
UniProt Secondary Accession:	Q8NEM0,Q66GU1, Q9H9C7, B4DWW2, E9PGU5, E9PH63,
UniProt Related Accession:	Q8NEM0
Molecular Weight:	835
NCBI Full Name:	Microcephalin
NCBI Synonym Full Names:	microcephalin 1
NCBI Official Symbol:	MCPH1
NCBI Official Synonym Symbols:	MCT; BRIT1
NCBI Protein Information:	microcephalin; BRCT-repeat inhibitor of TERT expression 1
UniProt Protein Name:	Microcephalin
Protein Family:	Microcephalin
UniProt Gene Name:	MCPH1
UniProt Entry Name:	MCPH1_HUMAN

Western blot analysis of extracts of various cell lines, using MCPH1 antibody (CAB5472) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Antibodies

MCPH1 Antibody (PACO05370)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)