Cell Cycle Antibodies 1

Anti-MCMBP Antibody (CAB14149)

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SKU:
CAB14149
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle

Description

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Antibody Name:Anti-MCMBP Antibody
Antibody SKU:CAB14149
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human MCMBP (NP_079110.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:A-431, Jurkat, Mouse spleen, Mouse liver, Mouse ovary, Rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human MCMBP (NP_079110.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MPCG EDWL SHPL GIVQ GFFA QNGV NPDW EKKV IEYF KEKL KENN APKW VPSL NEVP LHYL KPNS FVKF RCMI QDMF DPEF YMGV YETV NQNT KAHV LHFG KYRD VAEC GPQQ ELDL NSPR NTTL ERQT FYCV PVPG ESTW VKEA YVNA NQAR VSPS TSYT PSRH KRSY EDDD DMDL QPNK QKDQ HAGA RQAG SVGG LQWC GEPK RLET EAST GQQL NSLN LSSP FDLN FPLP GEKG PACL
Gene ID:79892
Uniprot:Q9BTE3
Cellular Location:Nucleus
Calculated MW:52kDa/72kDa
Observed MW:72kDa
Synonyms:MCMBP, C10orf119, MCM-BP
Background:This gene encodes a protein which is a component of the hexameric minichromosome maintenance (MCM) complex which regulates initiation and elongation of DNA. Multiple transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:MCMBP: Associated component of the MCM complex that acts as a regulator of DNA replication. Binds to the MCM complex during late S phase and promotes the disassembly of the MCM complex from chromatin, thereby acting as a key regulator of pre-replication complex (pre-RC) unloading from replicated DNA. Can dissociate the MCM complex without addition of ATP; probably acts by destabilizing interactions of each individual subunits of the MCM complex. Required for sister chromatid cohesion. Belongs to the MCMBP family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Unknown function

Chromosomal Location of Human Ortholog: 10q26.11

Cellular Component: nucleoplasm; MCM complex; cytoplasm; plasma membrane; nucleus

Molecular Function:protein binding; chromatin binding

Biological Process: mitosis; cell division; sister chromatid cohesion; DNA-dependent DNA replication

NCBI Summary:This gene encodes a protein which is a component of the hexameric minichromosome maintenance (MCM) complex which regulates initiation and elongation of DNA. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
UniProt Code:Q9BTE3
NCBI GenInfo Identifier:71153001
NCBI Gene ID:79892
NCBI Accession:Q9BTE3.2
UniProt Related Accession:Q9BTE3
Molecular Weight:52kDa; 72kDa
NCBI Full Name:Mini-chromosome maintenance complex-binding protein
NCBI Synonym Full Names:minichromosome maintenance complex binding protein
NCBI Official Symbol:MCMBP  
NCBI Official Synonym Symbols:MCM-BP; C10orf119  
NCBI Protein Information:mini-chromosome maintenance complex-binding protein
UniProt Protein Name:Mini-chromosome maintenance complex-binding protein
Protein Family:Mini-chromosome maintenance complex-binding protein
UniProt Gene Name:MCMBP  
UniProt Entry Name:MCMBP_HUMAN
Anti-MCMBP Antibody (CAB14149)Western blot analysis of extracts of various cell lines, using MCMBP antibody (CAB14149) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
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