Cell Cycle Antibodies 1

Anti-MCM5 Antibody (CAB5556)

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SKU:
CAB5556
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle

Description

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Antibody Name:Anti-MCM5 Antibody
Antibody SKU:CAB5556
Antibody Size:20uL, 50uL, 100uL
Application:WB IF IP
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human MCM5 (NP_006730.2).
Application:WB IF IP
Recommended Dilution:WB 1:1000 - 1:3000 IF 1:50 - 1:200 IP 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, Jurkat, SW620, Mouse spleen, Rat thymus
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human MCM5 (NP_006730.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MSGF DDPG IFYS DSFG GDAQ ADEG QARK SQLQ RRFK EFLR QYRV GTDR TGFT FKYR DELK RHYN LGEY WIEV EMED LASF DEDL ADYL YKQP AEHL QLLE EAAK EVAD EVTR PRPS GEEV LQDI QVML KSDA SPSS IRSL KSDM MSHL VKIP GIII AASA VRAK ATRI SIQC RSCR NTLT NIAM RPGL EGYA LPRK CNTD QAGR PKCP LDPY FIMP DKCK CVDF QTLK LQ
Gene ID:4174
Uniprot:P33992
Cellular Location:Nucleus
Calculated MW:82kDa
Observed MW:100kDa
Synonyms:MCM5, CDC46, P1-CDC46
Background:The protein encoded by this gene is structurally very similar to the CDC46 protein from S. cerevisiae, a protein involved in the initiation of DNA replication. The encoded protein is a member of the MCM family of chromatin-binding proteins and can interact with at least two other members of this family. The encoded protein is upregulated in the transition from the G0 to G1/S phase of the cell cycle and may actively participate in cell cycle regulation.
UniProt Protein Function:MCM5: Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Interacts with MCMBP. Belongs to the MCM family.
UniProt Protein Details:

Protein type:Cell cycle regulation; EC 3.6.4.12

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: nucleoplasm; MCM complex; membrane; nucleus

Molecular Function:DNA helicase activity; protein binding; DNA binding; ATP binding

Biological Process: DNA replication initiation; DNA strand elongation during DNA replication; mitotic cell cycle; DNA replication; DNA duplex unwinding; G1/S transition of mitotic cell cycle

NCBI Summary:The protein encoded by this gene is structurally very similar to the CDC46 protein from S. cerevisiae, a protein involved in the initiation of DNA replication. The encoded protein is a member of the MCM family of chromatin-binding proteins and can interact with at least two other members of this family. The encoded protein is upregulated in the transition from the G0 to G1/S phase of the cell cycle and may actively participate in cell cycle regulation. [provided by RefSeq, Jul 2008]
UniProt Code:P33992
NCBI GenInfo Identifier:19858646
NCBI Gene ID:4174
NCBI Accession:P33992.5
UniProt Secondary Accession:P33992,O60785, Q14578, Q9BTJ4, Q9BWL8,
UniProt Related Accession:P33992
Molecular Weight:82,286 Da
NCBI Full Name:DNA replication licensing factor MCM5
NCBI Synonym Full Names:minichromosome maintenance complex component 5
NCBI Official Symbol:MCM5  
NCBI Official Synonym Symbols:CDC46; P1-CDC46  
NCBI Protein Information:DNA replication licensing factor MCM5; CDC46 homolog; minichromosome maintenance deficient 5 (cell division cycle 46); MCM5 minichromosome maintenance deficient 5, cell division cycle 46
UniProt Protein Name:DNA replication licensing factor MCM5
UniProt Synonym Protein Names:CDC46 homolog; P1-CDC46
Protein Family:Minichromosome maintenance protein
UniProt Gene Name:MCM5  
UniProt Entry Name:MCM5_HUMAN
Anti-MCM5 Antibody (CAB5556)Western blot analysis of extracts of various cell lines, using MCM5 antibody (CAB5556) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-MCM5 Antibody (CAB5556)Immunofluorescence analysis of U-2 OS cells using MCM5 Rabbit pAb (CAB5556) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Anti-MCM5 Antibody (CAB5556)Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug MCM5 antibody (CAB5556). Western blot was performed from the immunoprecipitate using MCM5 antibody (CAB5556) at a dilition of 1:1000.
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