Cell Cycle Antibodies 1
Anti-MCM4 Antibody (CAB3018)
- SKU:
- CAB3018
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Cycle
Description
| Antibody Name: | Anti-MCM4 Antibody |
| Antibody SKU: | CAB3018 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide of human MCM4 |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Jurkat, K-562, Mouse thymus |
| Immunogen: | A synthetic peptide of human MCM4 |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 4173 |
| Uniprot: | P33991 |
| Cellular Location: | Nucleus |
| Calculated MW: | 96kDa |
| Observed MW: | 105kDa |
| Synonyms: | MCM4, CDC21, CDC54, NKCD, NKGCD, P1-CDC21, hCdc21 |
| Background: | The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported. |
| UniProt Protein Function: | MCM4: a mini-chromosome maintenance protein, essential for the initiation of eukaryotic genome replication. The MCM hexameric protein complex is a key component of the pre-replication complex. The complex consisting of this protein and MCM2, 6 and 7 possesses DNA helicase activity. Phosphorylation by CDC2 reduces the DNA helicase activity and chromatin binding of the complex. |
| UniProt Protein Details: | Protein type:EC 3.6.4.12; DNA replication Chromosomal Location of Human Ortholog: 8q11.2 Cellular Component: MCM complex; membrane; nuclear chromosome, telomeric region; nucleoplasm Molecular Function:ATP-dependent DNA helicase activity; protein binding Biological Process: DNA replication; G1/S transition of mitotic cell cycle Disease: Natural Killer Cell And Glucocorticoid Deficiency With Dna Repair Defect |
| NCBI Summary: | The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P33991 |
| NCBI GenInfo Identifier: | 68571766 |
| NCBI Gene ID: | 4173 |
| NCBI Accession: | P33991.5 |
| UniProt Secondary Accession: | P33991,Q8NEH1, Q99658, |
| UniProt Related Accession: | P33991 |
| Molecular Weight: | 96,558 Da |
| NCBI Full Name: | DNA replication licensing factor MCM4 |
| NCBI Synonym Full Names: | minichromosome maintenance complex component 4 |
| NCBI Official Symbol: | MCM4 |
| NCBI Official Synonym Symbols: | NKCD; CDC21; CDC54; NKGCD; hCdc21; P1-CDC21 |
| NCBI Protein Information: | DNA replication licensing factor MCM4 |
| UniProt Protein Name: | DNA replication licensing factor MCM4 |
| UniProt Synonym Protein Names: | CDC21 homolog; P1-CDC21 |
| Protein Family: | DNA replication licensing factor |
| UniProt Gene Name: | MCM4 |
| UniProt Entry Name: | MCM4_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MCM4 antibody (CAB3018) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded rat pancreas using MCM4 Antibody (CAB3018) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human gastric cancer using MCM4 Antibody (CAB3018) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse testis using MCM4 Antibody (CAB3018) at dilution of 1:100 (40x lens). |
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