Cell Cycle Antibodies 1

Anti-MCM4 Antibody (CAB3018)

(No reviews yet) Write a Review
SKU:
CAB3018
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Cycle

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-MCM4 Antibody
Antibody SKU:CAB3018
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide of human MCM4
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:Jurkat, K-562, Mouse thymus
Immunogen:A synthetic peptide of human MCM4
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:4173
Uniprot:P33991
Cellular Location:Nucleus
Calculated MW:96kDa
Observed MW:105kDa
Synonyms:MCM4, CDC21, CDC54, NKCD, NKGCD, P1-CDC21, hCdc21
Background:The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported.
UniProt Protein Function:MCM4: a mini-chromosome maintenance protein, essential for the initiation of eukaryotic genome replication. The MCM hexameric protein complex is a key component of the pre-replication complex. The complex consisting of this protein and MCM2, 6 and 7 possesses DNA helicase activity. Phosphorylation by CDC2 reduces the DNA helicase activity and chromatin binding of the complex.
UniProt Protein Details:

Protein type:EC 3.6.4.12; DNA replication

Chromosomal Location of Human Ortholog: 8q11.2

Cellular Component: MCM complex; membrane; nuclear chromosome, telomeric region; nucleoplasm

Molecular Function:ATP-dependent DNA helicase activity; protein binding

Biological Process: DNA replication; G1/S transition of mitotic cell cycle

Disease: Natural Killer Cell And Glucocorticoid Deficiency With Dna Repair Defect

NCBI Summary:The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008]
UniProt Code:P33991
NCBI GenInfo Identifier:68571766
NCBI Gene ID:4173
NCBI Accession:P33991.5
UniProt Secondary Accession:P33991,Q8NEH1, Q99658,
UniProt Related Accession:P33991
Molecular Weight:96,558 Da
NCBI Full Name:DNA replication licensing factor MCM4
NCBI Synonym Full Names:minichromosome maintenance complex component 4
NCBI Official Symbol:MCM4  
NCBI Official Synonym Symbols:NKCD; CDC21; CDC54; NKGCD; hCdc21; P1-CDC21  
NCBI Protein Information:DNA replication licensing factor MCM4
UniProt Protein Name:DNA replication licensing factor MCM4
UniProt Synonym Protein Names:CDC21 homolog; P1-CDC21
Protein Family:DNA replication licensing factor
UniProt Gene Name:MCM4  
UniProt Entry Name:MCM4_HUMAN
Anti-MCM4 Antibody (CAB3018)Western blot analysis of extracts of various cell lines, using MCM4 antibody (CAB3018) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-MCM4 Antibody (CAB3018)Immunohistochemistry of paraffin-embedded rat pancreas using MCM4 Antibody (CAB3018) at dilution of 1:100 (40x lens).
Anti-MCM4 Antibody (CAB3018)Immunohistochemistry of paraffin-embedded human gastric cancer using MCM4 Antibody (CAB3018) at dilution of 1:100 (40x lens).
Anti-MCM4 Antibody (CAB3018)Immunohistochemistry of paraffin-embedded mouse testis using MCM4 Antibody (CAB3018) at dilution of 1:100 (40x lens).
View AllClose

0 Reviews

View AllClose

Related Products