Name: Anti-MCM4 Antibody (CAB13513)
Brand: Cell Cycle Antibodies 1
SKU: CAB13513
Availability: OutOfStock

Antibody Name:	Anti-MCM4 Antibody
Antibody SKU:	CAB13513
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 350-450 of human MCM4 (NP_877423.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:100 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, Jurkat, Raji

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 350-450 of human MCM4 (NP_877423.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	QMIK LQES PEDM PAGQ TPHT VILF AHND LVDK VQPG DRVN VTGI YRAV PIRV NPRV SNVK SVYK THID VIHY RKTD AKRL HGLD EEAE QKLF SEKR VELL K
Gene ID:	4173
Uniprot:	P33991
Cellular Location:	Nucleus
Calculated MW:	96kDa
Observed MW:	110kDa

Synonyms:

MCM4, CDC21, CDC54, NKCD, NKGCD, P1-CDC21, hCdc21

Background:

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported.

UniProt Protein Function:	MCM4: a mini-chromosome maintenance protein, essential for the initiation of eukaryotic genome replication. The MCM hexameric protein complex is a key component of the pre-replication complex. The complex consisting of this protein and MCM2, 6 and 7 possesses DNA helicase activity. Phosphorylation by CDC2 reduces the DNA helicase activity and chromatin binding of the complex.
UniProt Protein Details:	Protein type:EC 3.6.4.12; DNA replication Chromosomal Location of Human Ortholog: 8q11.2 Cellular Component: MCM complex; membrane; nuclear chromosome, telomeric region; nucleoplasm Molecular Function:ATP-dependent DNA helicase activity; protein binding Biological Process: DNA replication; G1/S transition of mitotic cell cycle Disease: Natural Killer Cell And Glucocorticoid Deficiency With Dna Repair Defect
NCBI Summary:	The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008]
UniProt Code:	P33991
NCBI GenInfo Identifier:	68571766
NCBI Gene ID:	4173
NCBI Accession:	P33991.5
UniProt Secondary Accession:	P33991,Q8NEH1, Q99658,
UniProt Related Accession:	P33991
Molecular Weight:	96,558 Da
NCBI Full Name:	DNA replication licensing factor MCM4
NCBI Synonym Full Names:	minichromosome maintenance complex component 4
NCBI Official Symbol:	MCM4
NCBI Official Synonym Symbols:	NKCD; CDC21; CDC54; NKGCD; hCdc21; P1-CDC21
NCBI Protein Information:	DNA replication licensing factor MCM4
UniProt Protein Name:	DNA replication licensing factor MCM4
UniProt Synonym Protein Names:	CDC21 homolog; P1-CDC21
Protein Family:	DNA replication licensing factor
UniProt Gene Name:	MCM4
UniProt Entry Name:	MCM4_HUMAN

Western blot analysis of extracts of various cell lines, using MCM4 antibody (CAB13513) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.

Hong et al.

ITRAQ-based quantitative proteomic analysis reveals that VPS35 promotes the expression of MCM2-7 genes in HeLa cells

Scientific Reports 2022

PubMed ID: 35690672

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