Immunology Antibodies 2

Anti-MCM3AP Antibody (CAB15747)

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SKU:
CAB15747
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology

Description

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Antibody Name:Anti-MCM3AP Antibody
Antibody SKU:CAB15747
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 250-550 of human MCM3AP (NP_003897.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:Jurkat, HeLa, COS-7, NIH/3T3, Mouse brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 250-550 of human MCM3AP (NP_003897.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:SSFP VSSA VLGE PFQA SKAG VRQG CEEA VSQV EPLP SLMK GLKR KEDQ DRSP RRHG HEPA EDSD PLSR GDHP PDKR PVRL NRPR GGTL FGRT IQDV FKSN KEVG RLGN KEAK KETG FVES AESD HMAI PGGN QSVL APSR IPGV NKEE ETES REKK EDSL RGTP ARQS NRSE STDS LGGL SPSE VTAI QCKN IPDY LNDR TILE NHFG KIAK VQRI FTRR SKKL AVVH FFDH ASAA LARK KGKS LHKD MAIF WHRK KISP NKKP FSLK EKKP GDGE VSPS TEDA PFQH SPLG KAAG RTGA S
Gene ID:8888
Uniprot:O60318
Cellular Location:Cytoplasm, Nucleus, Nucleus envelope, nuclear pore complex, nucleoplasm
Calculated MW:80kDa/218kDa
Observed MW:70kDa
Synonyms:MCM3AP, GANP, MAP80, SAC3
Background:The minichromosome maintenance protein 3 (MCM3) is one of the MCM proteins essential for the initiation of DNA replication. The protein encoded by this gene is a MCM3 binding protein. It was reported to have phosphorylation-dependent DNA-primase activity, which was up-regulated in antigen immunization induced germinal center. This protein was demonstrated to be an acetyltransferase that acetylates MCM3 and plays a role in DNA replication. The mutagenesis of a nuclear localization signal of MCM3 affects the binding of this protein with MCM3, suggesting that this protein may also facilitate MCM3 nuclear localization. This gene is expressed in the brain or in neuronal tissue. An allelic variant encoding amino acid Lys at 915, instead of conserved Glu, has been identified in patients with mild intellectual disability.
UniProt Protein Function:GANP: is a member of the SAC3 family of nuclear export factors. In combination with ENY2, PCID2 and centrins, forms the TREX-2 complex that links transcription/ processing with nuclear mRNA export. GANP, required to recruit ENY2 to nuclear pore complexes (NPCs), requires both its CID and MCM3AP domains, together with nucleoporin Nup153, for recruitment to NPCs. The MCM3AP domain, corresponding to the carboxy-terminal 721 amino acids of GANP, shows homology with the Gcn5-related N acetyltransferase superfamily. The protein MCM3AP is encoded entirely within the 3¿ region of the GANP gene. MCM3AP transcription begins within intron 16 of GANP immediately upstream of the first MCM3AP exon, where NF-IL6, AP-1, NF-¿B, and p53 consensus transcription factor binding sites are located. In contrast, the GANP promoter region contains consensus transcription factor binding sites for E2F, E-box, BSAP, and PU.1. MCM3AP interacts with MCM3, which it can acetylate weakly in vivo. GANP is required for efficient mRNA nuclear export: GANP depletion results in the nuclear accumulation of poly(A)+RNA. It interacts via its N-terminal FG repeat domain with the mRNA export factor NXF1. GANP plays a role in the immune response: antibodies from mice deficient for GANP have reduced affinities against T-cell dependent antigens and a lower frequency of variable region somatic mutation. GANP expression is markedly decreased in malignant glioma (MG) with a poor prognosis, consistently lower than that observed in anaplastic astrocytomas. Significant decreases in GANP expression are associated with hyperploidy and chromosomal instability
UniProt Protein Details:

Protein type:Acetyltransferase; Karyopherin

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: nucleoplasm; nuclear membrane; cytoplasm; nuclear pore; nucleus; cytosol

Molecular Function:DNA binding; transferase activity, transferring acyl groups

Biological Process: mRNA transport; protein import into nucleus; immune system process; DNA replication

NCBI Summary:The minichromosome maintenance protein 3 (MCM3) is one of the MCM proteins essential for the initiation of DNA replication. The protein encoded by this gene is a MCM3 binding protein. It was reported to have phosphorylation-dependent DNA-primase activity, which was up-regulated in antigen immunization induced germinal center. This protein was demonstrated to be an acetyltransferase that acetylates MCM3 and plays a role in DNA replication. The mutagenesis of a nuclear localization signal of MCM3 affects the binding of this protein with MCM3, suggesting that this protein may also facilitate MCM3 nuclear localization. This gene is expressed in the brain or in neuronal tissue. An allelic variant encoding amino acid Lys at 915, instead of conserved Glu, has been identified in patients with mild intellectual disability. [provided by RefSeq, Jan 2014]
UniProt Code:O60318
NCBI GenInfo Identifier:8134564
NCBI Gene ID:8888
NCBI Accession:O60318.2
UniProt Secondary Accession:O60318,Q2M3C1, Q6PJP6, Q9BSY5, Q9UMT4, C9JL56,
UniProt Related Accession:O60318
Molecular Weight:80,292 Da
NCBI Full Name:Germinal-center associated nuclear protein
NCBI Synonym Full Names:minichromosome maintenance complex component 3 associated protein
NCBI Official Symbol:MCM3AP  
NCBI Official Synonym Symbols:GANP; SAC3; MAP80  
NCBI Protein Information:germinal-center associated nuclear protein; 80 kDa MCM3-associated protein; MCM3 acetylating protein; MCM3 acetyltransferase; MCM3 import protein; MCM3 minichromosome maintenance deficient 3 associated protein; germinal center-associated nuclear protein; germinal-centre associated nuclear protein
UniProt Protein Name:Germinal-center associated nuclear protein
UniProt Synonym Protein Names:80 kDa MCM3-associated protein; MCM3 acetylating protein (EC:2.3.1.-); MCM3AP; MCM3 acetyltransferase
Protein Family:Putative arabinogalactan oligomer transport system permease protein
UniProt Gene Name:MCM3AP  
UniProt Entry Name:GANP_HUMAN
Anti-MCM3AP Antibody (CAB15747)Western blot analysis of extracts of various cell lines, using MCM3AP antibody (CAB15747) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s.
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