Cell Cycle Antibodies 1
Anti-MCM3 Antibody (CAB1060)
- SKU:
- CAB1060
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Description
| Antibody Name: | Anti-MCM3 Antibody |
| Antibody SKU: | CAB1060 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF IP |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-295 of human MCM3 (NP_002379.2). |
| Application: | WB IHC IF IP |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, 293T, PC-12, NIH/3T3, COS-1, COS-7 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-295 of human MCM3 (NP_002379.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAGT VVLD DVEL REAQ RDYL DFLD DEED QGIY QSKV RELI SDNQ YRLI VNVN DLRR KNEK RANR LLNN AFEE LVAF QRAL KDFV ASID ATYA KQYE EFYV GLEG SFGS KHVS PRTL TSCF LSCV VCVE GIVT KCSL VRPK VVRS VHYC PATK KTIE RRYS DLTT LVAF PSSS VYPT KDEE NNPL ETEY GLSV YKDH QTIT IQEM PEKA PAGQ LPRS VDVI LDDD LVDK AKPG DRVQ VVGT YRCL PGKK GGYT SGTF RTVL IACN VKQM SKDA QPSF SAED IAKI KKFS KTRS KDI |
| Gene ID: | 4172 |
| Uniprot: | P25205 |
| Cellular Location: | Nucleus |
| Calculated MW: | 90kDa/95kDa |
| Observed MW: | 105kDa |
| Synonyms: | MCM3, HCC5, P1-MCM3, P1.h, RLFB |
| Background: | The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with and is acetylated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. Two transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | MCM3: a mini-chromosome maintenance protein, essential for the initiation of eukaryotic genome replication. Allows DNA to undergo a single round of replication per cell cycle. Required for DNA replication and cell proliferation. |
| UniProt Protein Details: | Protein type:EC 3.6.4.12; DNA replication Chromosomal Location of Human Ortholog: 6p12 Cellular Component: nucleoplasm; centrosome; alpha DNA polymerase:primase complex; membrane; intracellular membrane-bound organelle; MCM complex; perinuclear region of cytoplasm; nucleus Molecular Function:DNA helicase activity; protein binding; DNA binding; ATP binding Biological Process: DNA replication initiation; mitotic cell cycle; DNA strand elongation during DNA replication; DNA replication; DNA duplex unwinding; G1/S transition of mitotic cell cycle |
| NCBI Summary: | The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with and is acetylated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2012] |
| UniProt Code: | P25205 |
| NCBI GenInfo Identifier: | 19857543 |
| NCBI Gene ID: | 4172 |
| NCBI Accession: | P25205.3 |
| UniProt Secondary Accession: | P25205,Q92660, Q9BTR3, Q9NUE7, B4DWW4, |
| UniProt Related Accession: | P25205 |
| Molecular Weight: | 808 |
| NCBI Full Name: | DNA replication licensing factor MCM3 |
| NCBI Synonym Full Names: | minichromosome maintenance complex component 3 |
| NCBI Official Symbol: | MCM3 |
| NCBI Official Synonym Symbols: | HCC5; P1.h; RLFB; P1-MCM3 |
| NCBI Protein Information: | DNA replication licensing factor MCM3; p102; RLF subunit beta; hRlf beta subunit; DNA replication factor MCM3; cervical cancer proto-oncogene 5; minichromosome maintenance deficient 3; replication licensing factor, beta subunit; MCM3 minichromosome mainte |
| UniProt Protein Name: | DNA replication licensing factor MCM3 |
| UniProt Synonym Protein Names: | DNA polymerase alpha holoenzyme-associated protein P1; P1-MCM3; RLF subunit beta; p102 |
| Protein Family: | DNA replication licensing factor |
| UniProt Gene Name: | MCM3 |
| UniProt Entry Name: | MCM3_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MCM3 antibody (CAB1060) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunohistochemistry of paraffin-embedded human oophoroma using MCM3 antibody (CAB1060) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of U2OS cells using MCM3 antibody (CAB1060). Blue: DAPI for nuclear staining. |
 | Immunoprecipitation analysis of 300ug extracts of NIH/3T3 cells using 3ug MCM3 antibody (CAB1060). Western blot was performed from the immunoprecipitate using MCM3 antibody (CAB1060) at a dilition of 1:1000. |
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