Cell Cycle Antibodies 1
Anti-MCM2 Antibody (CAB1056)
- SKU:
- CAB1056
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Description
| Antibody Name: | Anti-MCM2 Antibody |
| Antibody SKU: | CAB1056 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 360-700 of human MCM2 (NP_004517.2). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:100 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | SW480, SH-SY5Y, Raji, A-549, HeLa, HL-60, Mouse thymus |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 360-700 of human MCM2 (NP_004517.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | PFEV NMEE TIYQ NYQR IRIQ ESPG KVAA GRLP RSKD AILL ADLV DSCK PGDE IELT GIYH NNYD GSLN TANG FPVF ATVI LANH VAKK DNKV AVGE LTDE DVKM ITSL SKDQ QIGE KIFA SIAP SIYG HEDI KRGL ALAL FGGE PKNP GGKH KVRG DINV LLCG DPGT AKSQ FLKY IEKV SSRA IFTT GQGA SAVG LTAY VQRH PVSR EWTL EAGA LVLA DRGV CLID EFDK MNDQ DRTS IHEA MEQQ SISI SKAG IVTS LQAR CTVI AAAN PIGG RYDP SLTF SENV DLTE PIIS RFDI LCVV RDTV DPVQ DEML ARFV VGSH VRHH PSNK EEEG LANG S |
| Gene ID: | 4171 |
| Uniprot: | P49736 |
| Cellular Location: | Nucleus |
| Calculated MW: | 101kDa |
| Observed MW: | 130kDa |
| Synonyms: | MCM2, BM28, CCNL1, CDCL1, D3S3194, DFNA70, MITOTIN, cdc19 |
| Background: | The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. |
| UniProt Protein Function: | MCM2: a mini-chromosome maintenance protein, essential for the initiation of eukaryotic genome replication. Allows DNA to undergo a single round of replication per cell cycle. Required for the entry in S phase and for cell division. |
| UniProt Protein Details: | Protein type:DNA-binding; EC 3.6.4.12 Chromosomal Location of Human Ortholog: 3q21 Cellular Component: microtubule cytoskeleton; nucleoplasm; MCM complex; cytoplasm; nuclear origin of replication recognition complex; nucleus; chromatin Molecular Function:protein binding; DNA helicase activity; DNA binding; histone binding; metal ion binding; DNA replication origin binding; ATP binding Biological Process: DNA unwinding during replication; nucleosome assembly; DNA replication initiation; DNA strand elongation during DNA replication; mitotic cell cycle; cell cycle; DNA replication; G1/S transition of mitotic cell cycle |
| NCBI Summary: | The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Oct 2012] |
| UniProt Code: | P49736 |
| NCBI GenInfo Identifier: | 41019490 |
| NCBI Gene ID: | 4171 |
| NCBI Accession: | P49736.4 |
| UniProt Secondary Accession: | P49736,Q14577, Q15023, Q8N2V1, Q969W7, Q96AE1, Q9BRM7 |
| UniProt Related Accession: | P49736 |
| Molecular Weight: | 904 |
| NCBI Full Name: | DNA replication licensing factor MCM2 |
| NCBI Synonym Full Names: | minichromosome maintenance complex component 2 |
| NCBI Official Symbol: | MCM2 |
| NCBI Official Synonym Symbols: | BM28; CCNL1; CDCL1; cdc19; D3S3194; MITOTIN |
| NCBI Protein Information: | DNA replication licensing factor MCM2; cyclin-like 1; nuclear protein BM28; cell devision cycle-like 1; minichromosome maintenance protein 2 homolog; minichromosome maintenance deficient 2 (mitotin); MCM2 minichromosome maintenance deficient 2, mitotin |
| UniProt Protein Name: | DNA replication licensing factor MCM2 |
| UniProt Synonym Protein Names: | Minichromosome maintenance protein 2 homolog; Nuclear protein BM28 |
| Protein Family: | DNA replication licensing factor |
| UniProt Gene Name: | MCM2 |
| UniProt Entry Name: | MCM2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MCM2 antibody (CAB1056) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunohistochemistry of paraffin-embedded human colon using MCM2 antibody (CAB1056) at dilution of 1:200 (40x lens). |
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