Antibody Name:	Anti-MCM10 Antibody
Antibody SKU:	CAB5427
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 616-875 of human MCM10 (NP_877428.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	Mouse heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 616-875 of human MCM10 (NP_877428.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	RLEG APAT MTPK LGRG VLEG DDVL FYDE SPPP RPKL SALA EAKK LAAI TKLR AKGQ VLTK TNPN SIKK KQKD PQDI LEVK ERVE KNTM FSSQ AEDE LEPA RKKR REQL AYLE SEEF QKIL KAKS KHTG ILKE AEAE MQER YFEP LVKK EQME EKMR NIRE VKCR VVTC KTCA YTHF KLLE TCVS EQHE YHWH DGVK RFFK CPCG NRSI SLDR LPNK HCSN CGLY KWER DGML KEKT GPKI GGET LLPR GEEH AKFL NSLK
Gene ID:	55388
Uniprot:	Q7L590
Cellular Location:	Nucleus
Calculated MW:	98kDa
Observed MW:	125kDa

Synonyms:

MCM10, CNA43, DNA43, PRO2249

Background:

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and it may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein can interact with MCM2 and MCM6, as well as with the origin recognition protein ORC2. It is regulated by proteolysis and phosphorylation in a cell cycle-dependent manner. Studies of a similar protein in Xenopus suggest that the chromatin binding of this protein at the onset of DNA replication is after pre-RC assembly and before origin unwinding. Alternatively spliced transcript variants encoding distinct isoforms have been identified.

UniProt Protein Function:	MCM10: Acts as a replication initiation factor that brings together the MCM2-7 helicase and the DNA polymerase alpha/primase complex in order to initiate DNA replication. Additionally, plays a role in preventing DNA damage during replication. Belongs to the MCM10 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:DNA replication Chromosomal Location of Human Ortholog: 10p13 Cellular Component: nucleoplasm; cytoplasm; nucleolus; nucleus Molecular Function:identical protein binding; protein binding; DNA binding; metal ion binding Biological Process: cell proliferation; mitotic cell cycle; DNA replication; G1/S transition of mitotic cell cycle
NCBI Summary:	The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and it may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein can interact with MCM2 and MCM6, as well as with the origin recognition protein ORC2. It is regulated by proteolysis and phosphorylation in a cell cycle-dependent manner. Studies of a similar protein in Xenopus suggest that the chromatin binding of this protein at the onset of DNA replication is after pre-RC assembly and before origin unwinding. Alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Jul 2008]
UniProt Code:	Q7L590
NCBI GenInfo Identifier:	126215746
NCBI Gene ID:	55388
NCBI Accession:	Q7L590.2
UniProt Secondary Accession:	Q7L590,Q3MIR3, Q7LD55, Q96GX4, Q96NB6, Q9H0D7, Q9H3P9 Q9P177, A8K9I6, B7ZKZ8,
UniProt Related Accession:	Q7L590,AAB34309,AAB34784
Molecular Weight:	875
NCBI Full Name:	Protein MCM10 homolog
NCBI Synonym Full Names:	minichromosome maintenance complex component 10
NCBI Official Symbol:	MCM10
NCBI Official Synonym Symbols:	CNA43; DNA43; PRO2249
NCBI Protein Information:	protein MCM10 homolog; hsMCM10; homolog of yeast MCM10; MCM10 minichromosome maintenance deficient 10
UniProt Protein Name:	Protein MCM10 homolog
Protein Family:	Minichromosome maintenance protein
UniProt Gene Name:	MCM10
UniProt Entry Name:	MCM10_HUMAN

	Western blot analysis of extracts of mouse heart, using MCM10 antibody (CAB5427) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunofluorescence analysis of A549 cells using MCM10 antibody (CAB5427). Blue: DAPI for nuclear staining.