Metabolism Antibodies 1
Anti-MCC Antibody (CAB13857)
- SKU:
- CAB13857
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-MCC Antibody |
| Antibody SKU: | CAB13857 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human MCC (NP_002378.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Jurkat, HT-29, Mouse liver, Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human MCC (NP_002378.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MNSG VAMK YGND SSAE LSEL HSAA LASL KGDI VELN KRLQ QTER ERDL LEKK LAKA QCEQ SHLM REHE DVQE RTTL RYEE RITE LHSV IAEL NKKI DRLQ GTTI REED EYSE LRSE LSQS QHEV NEDS RSMD QDQT SVSI PENQ STMV TADM DNCS DLNS ELQR VLTG LENV VCGR KKSS CSLS VAEV DRHI EQLT TASE HCDL AIKT VEEI EGVL GRDL YPNL AEER SRWE KELA GLRE ENES LTAM LCSK EEEL NRTK ATMN AIRE ERDR LRRR VREL QTRL QSVQ ATGP SSPG RLTS |
| Gene ID: | 4163 |
| Uniprot: | P23508 |
| Cellular Location: | Cell membrane, Cell projection, Cytoplasm, Nucleus, lamellipodium |
| Calculated MW: | 93kDa/113kDa |
| Observed MW: | 120kDa |
| Synonyms: | MCC, MCC1 |
| Background: | This gene is a candidate colorectal tumor suppressor gene that is thought to negatively regulate cell cycle progression. The orthologous gene in the mouse expresses a phosphoprotein associated with the plasma membrane and membrane organelles, and overexpression of the mouse protein inhibits entry into S phase. Multiple transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | MCC: Candidate for the putative colorectal tumor suppressor gene located at 5q21. Suppresses cell proliferation and the Wnt/b- catenin pathway in colorectal cancer cells. Inhibits DNA binding of b-catenin/TCF/LEF transcription factors. Involved in cell migration independently of RAC1, CDC42 and p21-activated kinase (PAK) activation. Belongs to the MCC family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Tumor suppressor; Cell cycle regulation Chromosomal Location of Human Ortholog: 5q21 Cellular Component: cytoplasm; nucleoplasm; nucleus; plasma membrane Molecular Function:protein binding; receptor activity Biological Process: establishment of protein localization; negative regulation of epithelial cell proliferation; signal transduction |
| NCBI Summary: | This gene is a candidate colorectal tumor suppressor gene that is thought to negatively regulate cell cycle progression. The orthologous gene in the mouse expresses a phosphoprotein associated with the plasma membrane and membrane organelles, and overexpression of the mouse protein inhibits entry into S phase. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P23508 |
| NCBI GenInfo Identifier: | 317373352 |
| NCBI Gene ID: | 4163 |
| NCBI Accession: | P23508.2 |
| UniProt Secondary Accession: | P23508,Q6ZR04, D3DT05, |
| UniProt Related Accession: | P23508 |
| Molecular Weight: | 113,279 Da |
| NCBI Full Name: | Colorectal mutant cancer protein |
| NCBI Synonym Full Names: | mutated in colorectal cancers |
| NCBI Official Symbol: | MCC |
| NCBI Official Synonym Symbols: | MCC1 |
| NCBI Protein Information: | colorectal mutant cancer protein |
| UniProt Protein Name: | Colorectal mutant cancer protein |
| Protein Family: | Colorectal mutant cancer protein |
| UniProt Gene Name: | MCC |
| UniProt Entry Name: | CRCM_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MCC antibody (CAB13857) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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