Name: Anti-MCAT Antibody (CAB15822)
Brand: Metabolism Antibodies 3
SKU: CAB15822
Availability: OutOfStock

Antibody Name:	Anti-MCAT Antibody
Antibody SKU:	CAB15822
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 191-390 of human MCAT (NP_775738.3).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	LO2

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 191-390 of human MCAT (NP_775738.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SGML SVLG QPQS KFNF ACLE AREH CKSL GIEN PVCE VSNY LFPD CRVI SGHQ EALR FLQK NSSK FHFR RTRM LPVS GAFH TRLM EPAV EPLT QALK AVDI KKPL VSVY SNVH AHRY RHPG HIHK LLAQ QLVS PVKW EQTM HAIY ERKK GRGF PQTF EVGP GRQL GAIL KSCN MQAW KSYS AVDV LQTL EHVD LDPQ EPPR
Gene ID:	27349
Uniprot:	Q8IVS2
Cellular Location:	Mitochondrion
Calculated MW:	19kDa/42kDa
Observed MW:	43kDa

Synonyms:

MCAT, FASN2C, MCT, MCT1, MT, NET62, fabD

Background:

The protein encoded by this gene is found exclusively in the mitochondrion, where it catalyzes the transfer of a malonyl group from malonyl-CoA to the mitochondrial acyl carrier protein. The encoded protein may be part of a fatty acid synthase complex that is more like the type II prokaryotic and plastid complexes rather than the type I human cytosolic complex. Alternative splicing results in multiple transcript variants encoding different isoforms.

UniProt Protein Function:	MCAT: Catalyzes the transfer of a malonyl moiety from malonyl- CoA to the free thiol group of the phosphopantetheine arm of the mitochondrial ACP protein (NDUFAB1). This suggests the existence of the biosynthesis of fatty acids in mitochondrias. Belongs to the type II malonyltransferase family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Lipid Metabolism - fatty acid biosynthesis; EC 2.3.1.39; Transferase Chromosomal Location of Human Ortholog: 22q13.31 Cellular Component: mitochondrion Molecular Function:[acyl-carrier-protein] S-malonyltransferase activity; transferase activity Biological Process: fatty acid biosynthetic process; metabolic process
NCBI Summary:	The protein encoded by this gene is found exclusively in the mitochondrion, where it catalyzes the transfer of a malonyl group from malonyl-CoA to the mitochondrial acyl carrier protein. The encoded protein may be part of a fatty acid synthase complex that is more like the type II prokaryotic and plastid complexes rather than the type I human cytosolic complex. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2012]
UniProt Code:	Q8IVS2
NCBI GenInfo Identifier:	48428076
NCBI Gene ID:	27349
NCBI Accession:	Q8IVS2.2
UniProt Secondary Accession:	Q8IVS2,O95510, O95511, B0QY72,
UniProt Related Accession:	Q8IVS2
Molecular Weight:	19,176 Da
NCBI Full Name:	Malonyl-CoA-acyl carrier protein transacylase, mitochondrial
NCBI Synonym Full Names:	malonyl-CoA-acyl carrier protein transacylase
NCBI Official Symbol:	MCAT
NCBI Official Synonym Symbols:	MT; MCT; fabD; NET62; FASN2C
NCBI Protein Information:	malonyl-CoA-acyl carrier protein transacylase, mitochondrial
UniProt Protein Name:	Malonyl-CoA-acyl carrier protein transacylase, mitochondrial
UniProt Synonym Protein Names:	Mitochondrial malonyl CoA:ACP acyltransferase; Mitochondrial malonyltransferase; [Acyl-carrier-protein] malonyltransferase
Protein Family:	Malonyl-CoA-acyl carrier protein transacylase
UniProt Gene Name:	MCAT
UniProt Entry Name:	FABD_HUMAN

Western blot analysis of extracts of LO2 cells, using MCAT antibody (CAB15822) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

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Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)