KO Validated Antibodies 2

Anti-MCAM Antibody (CAB19993)[KO Validated]

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SKU:
CAB19993
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-MCAM Antibody [KO Validated]
Antibody SKU:CAB19993
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant protein of human MCAM.
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:HeLa
Immunogen:Recombinant protein of human MCAM.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:4162
Uniprot:P43121
Cellular Location:
Calculated MW:57kDa/71kDa
Observed MW:120KDa
Anti-MCAM Antibody [KO Validated] (CAB19993)Western blot analysis of extracts from normal (control) and MCAM knockout (KO) HeLa cells, using MCAM antibody (CAB19993) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Synonyms:MCAM, CD146, MUC18
Background:
UniProt Protein Function:MCAM: Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q23.3

Cellular Component: extracellular space; focal adhesion; integral to membrane; plasma membrane; nucleus; external side of plasma membrane

Biological Process: anatomical structure morphogenesis; glomerular filtration; angiogenesis; cell adhesion; positive regulation of cell migration

UniProt Code:P43121
NCBI GenInfo Identifier:85681878
NCBI Gene ID:4162
NCBI Accession:P43121.2
UniProt Secondary Accession:P43121,O95812, Q59E86, Q6PHR3, Q6ZTR2,
UniProt Related Accession:P43121
Molecular Weight:646
NCBI Full Name:Cell surface glycoprotein MUC18
NCBI Synonym Full Names:melanoma cell adhesion molecule
NCBI Official Symbol:MCAM  
NCBI Official Synonym Symbols:CD146; MUC18  
NCBI Protein Information:cell surface glycoprotein MUC18; Gicerin; melanoma adhesion molecule; cell surface glycoprotein P1H12; melanoma-associated antigen A32; melanoma-associated antigen MUC18; S-endo 1 endothelial-associated antigen
UniProt Protein Name:Cell surface glycoprotein MUC18
UniProt Synonym Protein Names:Cell surface glycoprotein P1H12; Melanoma cell adhesion molecule; Melanoma-associated antigen A32; Melanoma-associated antigen MUC18; S-endo 1 endothelial-associated antigen; CD_antigen: CD146
Protein Family:Cell surface glycoprotein
UniProt Gene Name:MCAM  
UniProt Entry Name:MUC18_HUMAN
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