Metabolism Antibodies 3

Anti-MBTPS1 Antibody (CAB16243)

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SKU:
CAB16243
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

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Antibody Name:Anti-MBTPS1 Antibody
Antibody SKU:CAB16243
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 803-1052 of human MBTPS1 (NP_003782.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:K-562, Mouse pancreas
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 803-1052 of human MBTPS1 (NP_003782.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:ITQT FKDQ GLEV LKQE TAVV ENVP ILGL YQIP AEGG GRIV LYGD SNCL DDSH RQKD CFWL LDAL LQYT SYGV TPPS LSHS GNRQ RPPS GAGS VTPE RMEG NHLH RYSK VLEA HLGD PKPR PLPA CPRL SWAK PQPL NETA PSNL WKHQ KLLS IDLD KVVL PNFR SNRP QVRP LSPG ESGA WDIP GGIM PGRY NQEV GQTI PVFA FLGA MVVL AFFV VQIN KAKS RPKR RKPR VKRP QLMQ QVHP PKTP SV
Gene ID:8720
Uniprot:Q14703
Cellular Location:Endoplasmic reticulum membrane, Golgi apparatus membrane, Single-pass type I membrane protein
Calculated MW:117kDa
Observed MW:117kDa
Synonyms:MBTPS1, PCSK8, S1P, SKI-1
Background:This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. The encoded protein undergoes an initial autocatalytic processing event in the ER to generate a heterodimer which exits the ER and sorts to the cis/medial-Golgi where a second autocatalytic event takes place and the catalytic activity is acquired. It encodes a type 1 membrane bound protease which is ubiquitously expressed and regulates cholesterol or lipid homeostasis via cleavage of substrates at non-basic residues. Mutations in this gene may be associated with lysosomal dysfunction.
UniProt Protein Function:MBTPS1: Catalyzes the first step in the proteolytic activation of the sterol regulatory element-binding proteins (SREBPs). Other known substrates are BDNF and ATF6. Cleaves after hydrophobic or small residues, provided that Arg or Lys is in position P4. Cleaves known substrates after Arg-Ser-Val-Leu (SERBP-2), Arg-His- Leu-Leu (ATF6), Arg-Gly-Leu-Thr (BDNF) and its own propeptide after Arg-Arg-Leu-Leu. Belongs to the peptidase S8 family.
UniProt Protein Details:

Protein type:Membrane protein, integral; Endoplasmic reticulum; EC 3.4.21.112; Protease

Chromosomal Location of Human Ortholog: 16q24

Cellular Component: Golgi membrane; Golgi apparatus; endoplasmic reticulum membrane; Golgi stack; endoplasmic reticulum lumen; integral to membrane

Molecular Function:serine-type endopeptidase activity

Biological Process: cholesterol metabolic process; regulation of transcription factor import into nucleus; cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; membrane protein intracellular domain proteolysis; lysosome organization and biogenesis; unfolded protein response; lipid metabolic process; proteolysis

NCBI Summary:This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. The encoded protein undergoes an initial autocatalytic processing event in the ER to generate a heterodimer which exits the ER and sorts to the cis/medial-Golgi where a second autocatalytic event takes place and the catalytic activity is acquired. It encodes a type 1 membrane bound protease which is ubiquitously expressed and regulates cholesterol or lipid homeostasis via cleavage of substrates at non-basic residues. Mutations in this gene may be associated with lysosomal dysfunction. [provided by RefSeq, Feb 2014]
UniProt Code:Q14703
NCBI GenInfo Identifier:17368466
NCBI Gene ID:8720
NCBI Accession:Q14703.1
UniProt Secondary Accession:Q14703,Q24JQ2, Q9UF67, A8K6V8,
UniProt Related Accession:Q14703
Molecular Weight:
NCBI Full Name:Membrane-bound transcription factor site-1 protease
NCBI Synonym Full Names:membrane-bound transcription factor peptidase, site 1
NCBI Official Symbol:MBTPS1  
NCBI Official Synonym Symbols:S1P; PCSK8; SKI-1  
NCBI Protein Information:membrane-bound transcription factor site-1 protease; endopeptidase S1P; subtilisin/kexin isozyme-1; proprotein convertase subtilisin/kexin type 8
UniProt Protein Name:Membrane-bound transcription factor site-1 protease
UniProt Synonym Protein Names:Endopeptidase S1P; Subtilisin/kexin-isozyme 1; SKI-1
Protein Family:Membrane-bound transcription factor site-1 protease
UniProt Gene Name:MBTPS1  
UniProt Entry Name:MBTP1_HUMAN
Anti-MBTPS1 Antibody (CAB16243)Western blot analysis of extracts of various cell lines, using MBTPS1 antibody (CAB16243) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
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