Epigenetics & Nuclear Signaling Antibodies 1

Anti-MBNL2 Antibody (CAB12838)

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SKU:
CAB12838
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-MBNL2 Antibody
Antibody SKU:CAB12838
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 160-260 of human MBNL2 (NP_659002.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:A-549, HeLa, Mouse brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 160-260 of human MBNL2 (NP_659002.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:PPVT VPGS TATQ KLLR TDKL EVCR EFQR GNCA RGET DCRF AHPA DSTM IDTS DNTV TVCM DYIK GRCM REKC KYFH PPAH LQAK IKAA QHQA NQAA VAAQ A
Gene ID:10150
Uniprot:Q5VZF2
Cellular Location:Cytoplasm, Nucleus
Calculated MW:39kDa/40kDa
Observed MW:39kDa
Synonyms:MBNL2, MBLL, MBLL39, PRO2032
Background:This gene is a member of the muscleblind protein family which was initially described in Drosophila melanogaster. This gene encodes a C3H-type zinc finger protein that modulates alternative splicing of pre-mRNAs. Muscleblind proteins bind specifically to expanded dsCUG RNA but not to normal size CUG repeats and may thereby play a role in the pathophysiology of myotonic dystrophy. Several alternatively spliced transcript variants have been described but the full-length natures of only some have been determined.
UniProt Protein Function:MBNL2: Mediates pre-mRNA alternative splicing regulation. Acts either as activator or repressor of splicing on specific pre-mRNA targets. Inhibits cardiac troponin-T (TNNT2) pre-mRNA exon inclusion but induces insulin receptor (IR) pre-mRNA exon inclusion in muscle. Antagonizes the alternative splicing activity pattern of CELF proteins. RNA-binding protein that binds to 5'ACACCC-3' core sequence, termed zipcode, within the 3'UTR of ITGA3. Binds to CUG triplet repeat expansion in myotonic dystrophy muscle cells by sequestering the target RNAs. Seems to regulate expression and localization of ITGA3 by transporting it from the nucleus to cytoplasm at adhesion plaques. May play a role in myotonic dystrophy pathophysiology (DM). Belongs to the muscleblind family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:RNA-binding

Chromosomal Location of Human Ortholog: 13q32.1

Biological Process: regulation of RNA splicing

NCBI Summary:This gene is a member of the muscleblind protein family which was initially described in Drosophila melanogaster. This gene encodes a C3H-type zinc finger protein that modulates alternative splicing of pre-mRNAs. Muscleblind proteins bind specifically to expanded dsCUG RNA but not to normal size CUG repeats and may thereby play a role in the pathophysiology of myotonic dystrophy. Several alternatively spliced transcript variants have been described but the full-length natures of only some have been determined. [provided by RefSeq, Mar 2012]
UniProt Code:Q5VZF2
NCBI GenInfo Identifier:125952110
NCBI Gene ID:10150
NCBI Accession:Q5VZF2.2
UniProt Secondary Accession:Q5VZF2,Q3SXY5, Q58F19, Q8NEV3, Q8TD82,
UniProt Related Accession:Q5VZF2
Molecular Weight:39,356 Da
NCBI Full Name:Muscleblind-like protein 2
NCBI Synonym Full Names:muscleblind like splicing regulator 2
NCBI Official Symbol:MBNL2  
NCBI Official Synonym Symbols:MBLL; MBLL39; PRO2032  
NCBI Protein Information:muscleblind-like protein 2
UniProt Protein Name:Muscleblind-like protein 2
UniProt Synonym Protein Names:Muscleblind-like protein 1; Muscleblind-like protein-like; Muscleblind-like protein-like 39
Protein Family:Muscleblind-like protein
UniProt Gene Name:MBNL2  
UniProt Entry Name:MBNL2_HUMAN
Anti-MBNL2 Antibody (CAB12838)Western blot analysis of extracts of various cell lines, using MBNL2 antibody (CAB12838) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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