Epigenetics & Nuclear Signaling Antibodies 3
Anti-MBD3 Antibody (CAB2251)
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- SKU:
- CAB2251
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-MBD3 Antibody |
| Antibody SKU: | CAB2251 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-255 of human MBD3 (NP_001268383.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:200 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | Jurkat, K-562 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-255 of human MBD3 (NP_001268383.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MERK SPSG KKFR SKPQ LARY LGGS MDLS TFDF RTGK MLMS KMNK SRQR VRYD SSNQ VKGK PDLN TALP VRQT ASIF KQPV TKIT NHPS NKVK SDPQ KAVD QPRQ LFWE KKLS GLNA FDIA EELV KTMD LPKG LQGV GPGC TDET LLSA IASA LHTS TMPI TGQL SAAV EKNP GVWL NTTQ PLCK AFMV TDED IRKQ EELV QQVR KRLE EALM ADML AHVE ELAR DGEA PLDK ACAE DDDE EDEE EEEE EPDP DPE |
| Gene ID: | 53615 |
| Uniprot: | O95983 |
| Cellular Location: | Chromosome, Nucleus |
| Calculated MW: | 29kDa/32kDa |
| Observed MW: | 36kDa |
| Synonyms: | MBD3 |
| Background: | DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. This gene belongs to a family of nuclear proteins which are characterized by the presence of a methyl-CpG binding domain (MBD). The encoded protein is a subunit of the NuRD, a multisubunit complex containing nucleosome remodeling and histone deacetylase activities. Unlike the other family members, the encoded protein is not capable of binding to methylated DNA. The protein mediates the association of metastasis-associated protein 2 with the core histone deacetylase complex. Alternative splicing results in multiple transcript variants of this gene. |
| UniProt Protein Function: | MBD3: methyl-CpG binding (MBD) proteins appear to bind differentially methylated domains (DMDs) of DNA, to recruit repression complexes, and to silence the locus. The methyl CpG binding domain of human MBD3 may not directly bind mCpG but does interact with the NuRD/Mi2 components HDAC1 and MTA2. Plays a role in histone deacetylation and nucleosome remodeling. Increased levels have been reported in lung cancer and glioblastomas and may be a therapeutic target. |
| UniProt Protein Details: | Protein type:Gene silencing; Transcription, coactivator/corepressor Chromosomal Location of Human Ortholog: 19p13.3 Cellular Component: nuclear chromatin; nucleoplasm; protein complex Molecular Function:DNA binding; histone deacetylase activity; methyl-CpG binding; nucleosomal DNA binding; protein binding Biological Process: ATP-dependent chromatin remodeling; methylation-dependent chromatin silencing; negative regulation of transcription from RNA polymerase II promoter |
| NCBI Summary: | DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. This gene belongs to a family of nuclear proteins which are characterized by the presence of a methyl-CpG binding domain (MBD). The encoded protein is a subunit of the NuRD, a multisubunit complex containing nucleosome remodeling and histone deacetylase activities. Unlike the other family members, the encoded protein is not capable of binding to methylated DNA. The protein mediates the association of metastasis-associated protein 2 with the core histone deacetylase complex. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013] |
| UniProt Code: | O95983 |
| NCBI GenInfo Identifier: | 50400820 |
| NCBI Gene ID: | 53615 |
| NCBI Accession: | O95983.1 |
| UniProt Secondary Accession: | O95983,Q6PIL9, Q6PJZ9, Q86XF4, A8K4B7, D6W5Z2, |
| UniProt Related Accession: | O95983 |
| Molecular Weight: | 29,014 Da |
| NCBI Full Name: | Methyl-CpG-binding domain protein 3 |
| NCBI Synonym Full Names: | methyl-CpG binding domain protein 3 |
| NCBI Official Symbol: | MBD3 |
| NCBI Protein Information: | methyl-CpG-binding domain protein 3 |
| UniProt Protein Name: | Methyl-CpG-binding domain protein 3 |
| UniProt Synonym Protein Names: | Methyl-CpG-binding protein MBD3 |
| Protein Family: | Methyl-CpG-binding domain protein |
| UniProt Gene Name: | MBD3 |
| UniProt Entry Name: | MBD3_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MBD3 antibody (CAB2251) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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