Name: Anti-MBD2 Antibody (CAB2241)
Brand: Epigenetics & Nuclear Signaling Antibodies 3
SKU: CAB2241
Availability: OutOfStock

Antibody Name:	Anti-MBD2 Antibody
Antibody SKU:	CAB2241
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 202-411 of human MBD2 (NP_003918.1).

Application:	WB
Recommended Dilution:	WB 1:200 - 1:2000
Reactivity:	Mouse, Rat
Positive Samples:	mouse heart, mouse kidney, rat heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 202-411 of human MBD2 (NP_003918.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	DLSS FDFR TGKM MPSK LQKN KQRL RNDP LNQN KGKP DLNT TLPI RQTA SIFK QPVT KVTN HPSN KVKS DPQR MNEQ PRQL FWEK RLQG LSAS DVTE QIIK TMEL PKGL QGVG PGSN DETL LSAV ASAL HTSS APIT GQVS AAVE KNPA VWLN TSQP LCKA FIVT DEDI RKQE ERVQ QVRK KLEE ALMA DILS RAAD TEEM DIEM DSGD EA
Gene ID:	8932
Uniprot:	Q9UBB5
Cellular Location:	Nucleus
Calculated MW:	31kDa/43kDa
Observed MW:	53kDa

Synonyms:

MBD2, DMTase, NY-CO-41

Background:

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. The protein encoded by this gene may function as a mediator of the biological consequences of the methylation signal. It is also reported that the this protein functions as a demethylase to activate transcription, as DNA methylation causes gene silencing. Two transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	MBD2: Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binds hemimethylated DNA as well. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing. May enhance the activation of some unmethylated cAMP-responsive promoters. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Chromosomal Location of Human Ortholog: 18q21 Cellular Component: nucleoplasm; heterochromatin; protein complex; cytoplasm; nuclear chromatin; histone deacetylase complex; nucleus Molecular Function:mRNA binding; protein domain specific binding; protein binding; nucleosomal DNA binding; siRNA binding; methyl-CpG binding; satellite DNA binding Biological Process: cellular protein complex assembly; transcription, DNA-dependent; maternal behavior; negative regulation of gene expression, epigenetic; ATP-dependent chromatin remodeling; gene expression; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of Wnt receptor signaling pathway; regulation of gene expression, epigenetic; regulation of cell proliferation
NCBI Summary:	DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. The protein encoded by this gene may function as a mediator of the biological consequences of the methylation signal. It is also reported that the this protein functions as a demethylase to activate transcription, as DNA methylation causes gene silencing. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2011]
UniProt Code:	Q9UBB5
NCBI GenInfo Identifier:	50401198
NCBI Gene ID:	8932
NCBI Accession:	Q9UBB5.1
UniProt Related Accession:	Q9UBB5
Molecular Weight:	56kDa; 62kDa
NCBI Full Name:	Methyl-CpG-binding domain protein 2
NCBI Synonym Full Names:	methyl-CpG binding domain protein 2
NCBI Official Symbol:	MBD2
NCBI Official Synonym Symbols:	DMTase; NY-CO-41
NCBI Protein Information:	methyl-CpG-binding domain protein 2
UniProt Protein Name:	Methyl-CpG-binding domain protein 2
UniProt Synonym Protein Names:	Demethylase; DMTase; Methyl-CpG-binding protein MBD2
Protein Family:	Methyl-CpG-binding domain protein
UniProt Gene Name:	MBD2
UniProt Entry Name:	MBD2_HUMAN

Western blot analysis of extracts of various cell lines, using MBD2 antibody (CAB2241) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.

Antibodies

Anti-MBD2 Antibody (CAB11982)

MBD2 Antibody (PACO10405)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)