Epigenetics & Nuclear Signaling Antibodies 1

Anti-MAX Antibody (CAB0580)

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SKU:
CAB0580
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-MAX Antibody
Antibody SKU:CAB0580
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-1-160 of human MAX (NP_002373.3).
Application:WB
Recommended Dilution:WB 1:500 - 1:1000
Reactivity:Human
Positive Samples:U-87MG, HeLa
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-1-160 of human MAX (NP_002373.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MSDN DDIE VESD EEQP RFQS AADK RAHH NALE RKRR DHIK DSFH SLRD SVPS LQGE KASR AQIL DKAT EYIQ YMRR KNHT HQQD IDDL KRQN ALLE QQVR ALEK ARSS AQLQ TNYP SSDN SLYT NAKG STIS AFDG GSDS SSES EPEE PQSR KKLR MEAS
Gene ID:4149
Uniprot:P61244
Cellular Location:Cell projection, Nucleus, dendrite
Calculated MW:9kDa/11kDa/12kDa/15kDa/17kDa/18kDa
Observed MW:22kDa
Synonyms:MAX, bHLHd4
Background:The protein encoded by this gene is a member of the basic helix-loop-helix leucine zipper (bHLHZ) family of transcription factors. It is able to form homodimers and heterodimers with other family members, which include Mad, Mxi1 and Myc. Myc is an oncoprotein implicated in cell proliferation, differentiation and apoptosis. The homodimers and heterodimers compete for a common DNA target site (the E box) and rearrangement among these dimer forms provides a complex system of transcriptional regulation. Mutations of this gene have been reported to be associated with hereditary pheochromocytoma. A pseudogene of this gene is located on the long arm of chromosome 7. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:MAX: a transcription factor. Forms a DNA- binding protein complex with MYC or MAD. The MYC-MAX complex is a transcriptional activator, whereas the MAD-MAX complex is a repressor. CPG methylation of the recognition site greatly inhibits DNA binding, suggesting that DNA methylation may regulate the MYC/MAX complex in vivo. May repress transcription via the recruitment of a chromatin remodeling complex containing H3-K9 histone methyltransferase activity. Three alternatively spliced isoforms have been reported.
UniProt Protein Details:

Protein type:DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 14q23

Cellular Component: nucleoplasm; PML body; dendrite; cytoplasm; nucleus

Molecular Function:protein binding; protein homodimerization activity; sequence-specific DNA binding; protein heterodimerization activity; transcription coactivator activity; transcription cofactor activity; protein complex binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; neuron apoptosis; regulation of transcription, DNA-dependent; retina development in camera-type eye; protein complex assembly; response to axon injury; cellular response to starvation; response to insulin stimulus

NCBI Summary:The protein encoded by this gene is a member of the basic helix-loop-helix leucine zipper (bHLHZ) family of transcription factors. It is able to form homodimers and heterodimers with other family members, which include Mad, Mxi1 and Myc. Myc is an oncoprotein implicated in cell proliferation, differentiation and apoptosis. The homodimers and heterodimers compete for a common DNA target site (the E box) and rearrangement among these dimer forms provides a complex system of transcriptional regulation. Mutations of this gene have been reported to be associated with hereditary pheochromocytoma. A pseudogene of this gene is located on the long arm of chromosome 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]
UniProt Code:P61244
NCBI GenInfo Identifier:47117704
NCBI Gene ID:4149
NCBI Accession:P61244.1
UniProt Secondary Accession:P61244,P25912, P52163, Q14803, Q96CY8, A6NH73, A8K265 A8K4G4, A8K824,
UniProt Related Accession:P61244
Molecular Weight:160
NCBI Full Name:Protein max
NCBI Synonym Full Names:MYC associated factor X
NCBI Official Symbol:MAX  
NCBI Official Synonym Symbols:bHLHd4  
NCBI Protein Information:protein max; class D basic helix-loop-helix protein 4
UniProt Protein Name:Protein max
UniProt Synonym Protein Names:Class D basic helix-loop-helix protein 4; bHLHd4; Myc-associated factor X
Protein Family:Protein max
UniProt Gene Name:MAX  
UniProt Entry Name:MAX_HUMAN
Anti-MAX Antibody (CAB0580)Western blot analysis of extracts of various cell lines, using MAX antibody (CAB0580) at 1:3000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.
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