Metabolism Antibodies 3

Anti-MAT2A Antibody (CAB8436)

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SKU:
CAB8436
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Metabolism

Description

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Antibody Name:Anti-MAT2A Antibody
Antibody SKU:CAB8436
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-395 of human MAT2A (NP_005902.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:A-549, HeLa, SW620, Mouse lung, Mouse spleen, Rat kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-395 of human MAT2A (NP_005902.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MNGQ LNGF HEAF IEEG TFLF TSES VGEG HPDK ICDQ ISDA VLDA HLQQ DPDA KVAC ETVA KTGM ILLA GEIT SRAA VDYQ KVVR EAVK HIGY DDSS KGFD YKTC NVLV ALEQ QSPD IAQG VHLD RNEE DIGA GDQG LMFG YATD ETEE CMPL TIVL AHKL NAKL AELR RNGT LPWL RPDS KTQV TVQY MQDR GAVL PIRV HTIV ISVQ HDEE VCLD EMRD ALKE KVIK AVVP AKYL DEDT IYHL QPSG RFVI GGPQ GDAG LTGR KIIV DTYG GWGA HGGG AFSG KDYT KVDR SAAY AARW VAKS LVKG GLCR RVLV QVSY AIGV SHPL SISI FHYG TSQK SERE LLEI VKKN FDLR PGVI VRDL DLKK PIYQ RTAA YGHF GRDS FPWE VPKK LKY
Gene ID:4144
Uniprot:P31153
Cellular Location:
Calculated MW:32kDa/43kDa
Observed MW:44kDa
Synonyms:MAT2A, MATA2, MATII, SAMS2
Background:The protein encoded by this gene catalyzes the production of S-adenosylmethionine (AdoMet) from methionine and ATP. AdoMet is the key methyl donor in cellular processes.
UniProt Protein Function:MAT2A: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. Belongs to the AdoMet synthase family.
UniProt Protein Details:

Protein type:Transferase; Other Amino Acids Metabolism - selenoamino acid; Amino Acid Metabolism - cysteine and methionine; EC 2.5.1.6

Chromosomal Location of Human Ortholog: 2p11.2

Cellular Component: cytosol; methionine adenosyltransferase complex

Molecular Function:methionine adenosyltransferase activity; protein binding

Biological Process: methylation; S-adenosylmethionine biosynthetic process

NCBI Summary:The protein encoded by this gene catalyzes the production of S-adenosylmethionine (AdoMet) from methionine and ATP. AdoMet is the key methyl donor in cellular processes. [provided by RefSeq, Jun 2011]
UniProt Code:P31153
NCBI GenInfo Identifier:400245
NCBI Gene ID:4144
NCBI Accession:P31153.1
UniProt Secondary Accession:P31153,Q53SP5, A8K511, B4DN45, D6W5L1,
UniProt Related Accession:P31153
Molecular Weight:32,965 Da
NCBI Full Name:S-adenosylmethionine synthase isoform type-2
NCBI Synonym Full Names:methionine adenosyltransferase 2A
NCBI Official Symbol:MAT2A  
NCBI Official Synonym Symbols:MATA2; MATII; SAMS2  
NCBI Protein Information:S-adenosylmethionine synthase isoform type-2
UniProt Protein Name:S-adenosylmethionine synthase isoform type-2
UniProt Synonym Protein Names:Methionine adenosyltransferase 2; MAT 2; Methionine adenosyltransferase II; MAT-II
Protein Family:S-adenosylmethionine synthase
UniProt Gene Name:MAT2A  
UniProt Entry Name:METK2_HUMAN
Anti-MAT2A Polyclonal Antibody (CAB8436)Western blot analysis of extracts of various cell lines, using MAT2A antibody (CAB8436) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
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