Immunology Antibodies 2
Anti-MASP2 Antibody (CAB16030)
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- SKU:
- CAB16030
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Description
| Antibody Name: | Anti-MASP2 Antibody |
| Antibody SKU: | CAB16030 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 445-686 of human MASP2 (NP_006601.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HepG2, U-87MG, Mouse liver, Rat kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 445-686 of human MASP2 (NP_006601.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | IYGG QKAK PGDF PWQV LILG GTTA AGAL LYDN WVLT AAHA VYEQ KHDA SALD IRMG TLKR LSPH YTQA WSEA VFIH EGYT HDAG FDND IALI KLNN KVVI NSNI TPIC LPRK EAES FMRT DDIG TASG WGLT QRGF LARN LMYV DIPI VDHQ KCTA AYEK PPYP RGSV TANM LCAG LESG GKDS CRGD SGGA LVFL DSET ERWF VGGI VSWG SMNC GEAG QYGV YTKV INYI PWIE NIIS DF |
| Gene ID: | 10747 |
| Uniprot: | O00187 |
| Cellular Location: | |
| Calculated MW: | 20kDa/75kDa |
| Observed MW: | 76kDa |
| Synonyms: | MASP2, MAP19, MASP-2, MASP1P1, sMAP |
| Background: | This gene encodes a member of the peptidase S1 family of serine proteases. The encoded preproprotein is proteolytically processed to generate A and B chains that heterodimerize to form the mature protease. This protease cleaves complement components C2 and C4 in order to generate C3 convertase in the lectin pathway of the complement system. The encoded protease also plays a role in the coagulation cascade through cleavage of prothrombin to form thrombin. Myocardial infarction and acute stroke patients exhibit reduced serum concentrations of the encoded protein. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. |
| UniProt Protein Function: | MASP2: Serum protease that plays an important role in the activation of the complement system via mannose-binding lectin. After activation by auto-catalytic cleavage it cleaves C2 and C4, leading to their activation and to the formation of C3 convertase. Defects in MASP2 are the cause of MASP2 deficiency (MASPD). MASPD is a disorder that results in autoimmune manifestations, recurrent severe infections, and chronic inflammatory disease. Belongs to the peptidase S1 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 3.4.21.104; Protease; Secreted, signal peptide; Secreted Chromosomal Location of Human Ortholog: 1p36.3-p36.2 Cellular Component: extracellular region Molecular Function:peptidase activity; protein binding; serine-type endopeptidase activity; complement component C4b binding; calcium ion binding; calcium-dependent protein binding Biological Process: innate immune response; complement activation, lectin pathway; proteolysis; complement activation, classical pathway; complement activation Disease: Masp2 Deficiency |
| NCBI Summary: | The Ra-reactive factor (RARF) is a complement-dependent bactericidal factor that binds to the Ra and R2 polysaccharides expressed by certain enterobacteria. Alternate splicing of this gene results in two transcript variants encoding two RARF components that are involved in the mannan-binding lectin pathway of complement activation. The longer isoform is cleaved into two chains which form a heterodimer linked by a disulfide bond. The encoded proteins are members of the trypsin family of peptidases. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O00187 |
| NCBI GenInfo Identifier: | 21264363 |
| NCBI Gene ID: | 10747 |
| NCBI Accession: | NP_006601.2 |
| UniProt Secondary Accession: | O00187,O75754, Q5TEQ5, Q5TER0, Q96QG4, Q9BZH0, Q9H498 Q9H499, Q9UBP3, Q9UC48, A8K458, A8MWJ2, |
| UniProt Related Accession: | O00187 |
| Molecular Weight: | 76 kDa |
| NCBI Full Name: | mannan-binding lectin serine protease 2 isoform 1 preproprotein |
| NCBI Synonym Full Names: | mannan-binding lectin serine peptidase 2 |
| NCBI Official Symbol: | MASP2 |
| NCBI Official Synonym Symbols: | sMAP; MAP19; MASP-2; MASP1P1 |
| NCBI Protein Information: | mannan-binding lectin serine protease 2 |
| UniProt Protein Name: | Mannan-binding lectin serine protease 2 |
| UniProt Synonym Protein Names: | MBL-associated serine protease 2; Mannose-binding protein-associated serine protease 2; MASP-2 |
| Protein Family: | Mannan-binding lectin serine protease |
| UniProt Gene Name: | MASP2 |
| UniProt Entry Name: | MASP2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MASP2 antibody (CAB16030) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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