Antibody Name:	MAS1 Rabbit Polyclonal Antibody
Antibody SKU:	CAB8132
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 246-325 of human MAS1 (NP_002368.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HepG2, A-549

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 246-325 of human MAS1 (NP_002368.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LLYL LYYE YWST FGNL HHIS LLFS TINS SANP FIYF FVGS SKKK RFKE SLKV VLTR AFKD EMQP RRQK DNCN TVTV ETVV
Gene ID:	4142
Uniprot:	P04201
Cellular Location:	Cell membrane, Multi-pass membrane protein
Calculated MW:	37kDa
Observed MW:	41kDa

Synonyms:

MAS1, MAS, MGRA

Background:

This gene encodes a class I seven-transmembrane G-protein-coupled receptor. The encoded protein is a receptor for angiotensin-(1-7) and preferentially couples to the Gq protein, activating the phospholipase C signaling pathway. The encoded protein may play a role in multiple processes including hypotension, smooth muscle relaxation and cardioprotection by mediating the effects of angiotensin-(1-7).

UniProt Protein Function:	MAS1: Receptor for angiotensin 1-7. Acts specifically as a functional antagonist of AGTR1 (angiotensin-2 type 1 receptor), although it up-regulates AGTR1 receptor levels. Positive regulation of AGTR1 levels occurs through activation of the G-proteins GNA11 and GNAQ, and stimulation of the protein kinase C signaling cascade. The antagonist effect on AGTR1 function is probably due to AGTR1 being physically altered by MAS1. The MAS oncogene has a weak focus-inducing activity in transfected NIH 3T3 cells. Belongs to the G-protein coupled receptor 1 family.
UniProt Protein Details:	Protein type:GPCR, family 1; Oncoprotein; Receptor, GPCR; Membrane protein, integral; Membrane protein, multi-pass Chromosomal Location of Human Ortholog: 6q25.3-q26 Cellular Component: integral to plasma membrane; plasma membrane Molecular Function:angiotensin receptor activity; angiotensin type II receptor activity; G-protein coupled receptor activity; peptide binding; protein binding Biological Process: anatomical structure morphogenesis; cell proliferation; G-protein coupled receptor protein signaling pathway
NCBI Summary:	This gene encodes a class I seven-transmembrane G-protein-coupled receptor. The encoded protein is a receptor for angiotensin-(1-7) and preferentially couples to the Gq protein, activating the phospholipase C signaling pathway. The encoded protein may play a role in multiple processes including hypotension, smooth muscle relaxation and cardioprotection by mediating the effects of angiotensin-(1-7). [provided by RefSeq, May 2012]
UniProt Code:	P04201
NCBI GenInfo Identifier:	135920
NCBI Gene ID:	4142
NCBI Accession:	P04201.1
UniProt Secondary Accession:	P04201,Q2TBC9, Q6FG47, E1P5B3,
UniProt Related Accession:	P04201
Molecular Weight:	37,465 Da
NCBI Full Name:	Proto-oncogene Mas
NCBI Synonym Full Names:	MAS1 proto-oncogene, G protein-coupled receptor
NCBI Official Symbol:	MAS1
NCBI Official Synonym Symbols:	MAS; MGRA
NCBI Protein Information:	proto-oncogene Mas
UniProt Protein Name:	Proto-oncogene Mas
Protein Family:	Proto-oncogene
UniProt Gene Name:	MAS1
UniProt Entry Name:	MAS_HUMAN

	Western blot analysis of extracts of various cell lines, using MAS1 antibody (CAB8132) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded mouse kidney using MAS1 antibody (CAB8132) at dilution of 1:100 (40x lens).