Metabolism Antibodies 3
Anti-MARS Antibody (CAB9938)
- SKU:
- CAB9938
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-MARS Antibody |
| Antibody SKU: | CAB9938 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human MARS (NP_004981.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | SGC-7901, HepG2, HeLa, SW480, HT-1080 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human MARS (NP_004981.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MRLF VSDG VPGC LPVL AAAG RARG RAEV LIST VGPE DCVV PFLT RPKV PVLQ LDSG NYLF STSA ICRY FFLL SGWE QDDL TNQW LEWE ATEL QPAL SAAL YYLV VQGK KGED VLGS VRRA LTHI DHSL SRQN CPFL AGET ESLA DIVL WGAL YPLL QDPA YLPE ELSA LHSW FQTL STQE PCQR AAET VLKQ QGVL ALRP YLQK QPQP SPAE GRAV TNEP EEEE LATL SEEE IAMA VTAW EKGL ESLP PLRP QQNP VLPV AGER NVLI TS |
| Gene ID: | 4141 |
| Uniprot: | P56192 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 61kDa/101kDa |
| Observed MW: | 110kDa |
| Synonyms: | MARS, CMT2U, ILFS2, ILLD, METRS, MRS, MTRNS, SPG70 |
| Background: | This gene encodes a member of the class I family of aminoacyl-tRNA synthetases. These enzymes play a critical role in protein biosynthesis by charging tRNAs with their cognate amino acids. The encoded protein is a component of the multi-tRNA synthetase complex and catalyzes the ligation of methionine to tRNA molecules. |
| UniProt Protein Function: | MARS: a member of the class I family of aminoacyl-tRNA synthetases. These enzymes play a critical role in protein biosynthesis by charging tRNAs with their cognate amino acids. The encoded protein is a component of the multi-tRNA synthetase complex and catalyzes the ligation of methionine to tRNA molecules. [provided by RefSeq, Jan 2011] |
| UniProt Protein Details: | Protein type:Translation; EC 6.1.1.10; Ligase; Other Amino Acids Metabolism - selenoamino acid Chromosomal Location of Human Ortholog: 12q13.3 Cellular Component: cytoplasm; cytosol; membrane Molecular Function:methionine-tRNA ligase activity Biological Process: tRNA aminoacylation for protein translation Disease: Charcot-marie-tooth Disease, Axonal, Type 2u; Infantile Liver Failure Syndrome 2 |
| NCBI Summary: | This gene encodes a member of the class I family of aminoacyl-tRNA synthetases. These enzymes play a critical role in protein biosynthesis by charging tRNAs with their cognate amino acids. The encoded protein is a component of the multi-tRNA synthetase complex and catalyzes the ligation of methionine to tRNA molecules. [provided by RefSeq, Jan 2011] |
| UniProt Code: | P56192 |
| NCBI GenInfo Identifier: | 20178332 |
| NCBI Gene ID: | 4141 |
| NCBI Accession: | P56192.2 |
| UniProt Secondary Accession: | P56192,Q14895, Q53H14, Q96A15, Q96BZ0, Q9NSE0, B3KVK7 |
| UniProt Related Accession: | P56192 |
| Molecular Weight: | 61,816 Da |
| NCBI Full Name: | Methionine--tRNA ligase, cytoplasmic |
| NCBI Synonym Full Names: | methionyl-tRNA synthetase |
| NCBI Official Symbol: | MARS |
| NCBI Official Synonym Symbols: | MRS; ILLD; CMT2U; ILFS2; METRS; MTRNS; SPG70 |
| NCBI Protein Information: | methionine--tRNA ligase, cytoplasmic |
| UniProt Protein Name: | Methionine--tRNA ligase, cytoplasmic |
| UniProt Synonym Protein Names: | Methionyl-tRNA synthetase; MetRS |
| Protein Family: | Methionine--tRNA ligase |
| UniProt Gene Name: | MARS |
| UniProt Entry Name: | SYMC_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MARS antibody (CAB9938) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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