Cell Biology Antibodies 1
Anti-MARCKS Antibody (CAB0936)
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- SKU:
- CAB0936
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-MARCKS Antibody |
| Antibody SKU: | CAB0936 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MARCKS (NP_002347.5). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Rat brain |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MARCKS (NP_002347.5). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MGAQ FSKT AAKG EAAA ERPG EAAV ASSP SKAN GQEN GHVK VNGD ASPA AAES GAKE ELQA NGSA PAAD KEEP AAAG SGAA SPSA AEKG EPAA AAAP EAGA |
| Gene ID: | 4082 |
| Uniprot: | P29966 |
| Cellular Location: | Cytoplasm, Lipid-anchor, Membrane, cytoskeleton |
| Calculated MW: | 31kDa |
| Observed MW: | 80kDa |
| Synonyms: | MARCKS, 80K-L, MACS, PKCSL, PRKCSL |
| Background: | The protein encoded by this gene is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. The protein is thought to be involved in cell motility, phagocytosis, membrane trafficking and mitogenesis. |
| UniProt Protein Function: | MARCKS: the most prominent cellular substrate of protein kinase C. Binds calmodulin, actin, and synapsin. A filamentous actin cross-linking protein. Critical for normal brain development. Phosphorylation by PKC displaces it from the plasma membrane, inhibiting F-actin cross-linking activity. Plays a role in growth cone adhesion: its nonphosphorylated form stabilizes integrin-mediated adhesions, and its phosphorylation-triggered release from adhesions causes localized growth cone detachment. Highly expressed in immature T and B lymphocytes: its expression is highest in CD4-8- T cells and B-cell blasts generated by antigen receptor signaling. Its expression and phosphorylation are closely linked to microglial activation processes involved in pathological conditions such as neuroinflammation and neurodegeneration. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Actin-binding Chromosomal Location of Human Ortholog: 6q22.2 Cellular Component: actin cytoskeleton; cell cortex; centrosome; cytosol; dendritic spine; focal adhesion; germinal vesicle; growth cone; lysosome; microtubule; nerve terminal; outer dense fiber; plasma membrane Molecular Function:actin filament binding; calmodulin binding; phosphatidylserine binding; protein kinase C binding Biological Process: actin filament organization; brain development; cell-substrate adhesion; energy reserve metabolic process; intracellular protein transport; phospholipase D activation; regulation of insulin secretion |
| NCBI Summary: | The protein encoded by this gene is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. The protein is thought to be involved in cell motility, phagocytosis, membrane trafficking and mitogenesis. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P29966 |
| NCBI GenInfo Identifier: | 76803798 |
| NCBI Gene ID: | 4082 |
| NCBI Accession: | P29966.4 |
| UniProt Secondary Accession: | P29966,Q2LA83, Q5TDB7, E1P560, |
| UniProt Related Accession: | P29966 |
| Molecular Weight: | |
| NCBI Full Name: | Myristoylated alanine-rich C-kinase substrate |
| NCBI Synonym Full Names: | myristoylated alanine rich protein kinase C substrate |
| NCBI Official Symbol: | MARCKS |
| NCBI Official Synonym Symbols: | MACS; 80K-L; PKCSL; PRKCSL |
| NCBI Protein Information: | myristoylated alanine-rich C-kinase substrate |
| UniProt Protein Name: | Myristoylated alanine-rich C-kinase substrate |
| UniProt Synonym Protein Names: | Protein kinase C substrate, 80 kDa protein, light chain; 80K-L protein; PKCSL |
| Protein Family: | MARCKS-related protein |
| UniProt Gene Name: | MARCKS |
| UniProt Entry Name: | MARCS_HUMAN |
 | Western blot analysis of extracts of rat brain, using MARCKS antibody (CAB0936) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunofluorescence analysis of C6 cells using MARCKS Rabbit pAb (CAB0936) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of NIH-3T3 cells using MARCKS Rabbit pAb (CAB0936) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U-2 OS cells using MARCKS Rabbit pAb (CAB0936) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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