Signal Transduction Antibodies 1

Anti-MARCH9 Antibody (CAB10596)

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SKU:
CAB10596
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction

Description

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Antibody Name:Anti-MARCH9 Antibody
Antibody SKU:CAB10596
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 277-346 of human MARCH9 (NP_612405.2).
Application:WB
Recommended Dilution:WB 1:1000 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:U-251MG, A-549, 293T, Mouse intestine, Mouse kidney, Mouse eye, Rat kidney, Rat eye
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 277-346 of human MARCH9 (NP_612405.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:GTAG KSGP RNSR TGPT SGAT SRPP AAQR MRTL LPQR CGYT ILHL LGQL RPPD ARSS SHSG REVV MRVT TV
Gene ID:92979
Uniprot:Q86YJ5
Cellular Location:Golgi apparatus membrane, Lysosome membrane, Multi-pass membrane protein
Calculated MW:25kDa/37kDa
Observed MW:43kDa
Synonyms:MARCH9, MARCH-IX, RNF179
Background:
UniProt Protein Function:MARCH9: E3 ubiquitin-protein ligase that may mediate ubiquitination of MHC-I, CD4 and ICAM1, and promote their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 6.3.2.-; EC 6.3.2.19; Ligase; Membrane protein, integral; Membrane protein, multi-pass; Ubiquitin conjugating system; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 12q14.1

Cellular Component: Golgi stack; trans-Golgi network

NCBI Summary:MARCH9 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments. MARCH9 induces internalization of several membrane glycoproteins and directs them to the endosomal compartment (Bartee et al., 2004 [PubMed 14722266]; Hoer et al., 2007 [PubMed 17174307]).[supplied by OMIM, Apr 2010]
UniProt Code:Q86YJ5
NCBI GenInfo Identifier:74759533
NCBI Gene ID:92979
NCBI Accession:Q86YJ5.2
UniProt Secondary Accession:Q86YJ5,Q86VN5, Q96GG2, B2R9U9,
UniProt Related Accession:Q86YJ5
Molecular Weight:38kDa
NCBI Full Name:E3 ubiquitin-protein ligase MARCH9
NCBI Synonym Full Names:membrane associated ring-CH-type finger 9
NCBI Official Symbol:MARCH9  
NCBI Official Synonym Symbols:RNF179; MARCH-IX  
NCBI Protein Information:E3 ubiquitin-protein ligase MARCH9
UniProt Protein Name:E3 ubiquitin-protein ligase MARCH9
UniProt Synonym Protein Names:Membrane-associated RING finger protein 9; Membrane-associated RING-CH protein IX; MARCH-IX; RING finger protein 179; RING-type E3 ubiquitin transferase MARCH9Curated
UniProt Gene Name:MARCH9  
Anti-MARCH9 Antibody (CAB10596)Western blot analysis of extracts of various cell lines, using MARCH9 antibody (CAB10596) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
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