Immunology Antibodies 1

Anti-MARCH8 Antibody (CAB12962)

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SKU:
CAB12962
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology

Description

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Antibody Name:Anti-MARCH8 Antibody
Antibody SKU:CAB12962
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 232-291 of human MARCH8 (NP_659458.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:22Rv1, U-87MG, Mouse testis, Rat testis, Rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 232-291 of human MARCH8 (NP_659458.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:YNRV IYVQ NCPE TSKK NIFE KSPL TEPN FENK HGYG ICHS DTNS SCCT EPED TGAE IIHV
Gene ID:220972
Uniprot:Q5T0T0
Cellular Location:Cytoplasmic vesicle membrane, Early endosome membrane, Lysosome membrane, Multi-pass membrane protein
Calculated MW:32kDa/63kDa
Observed MW:33kDa
Synonyms:MARCH8, CMIR, MARCH-VIII, MIR, RNF178, c-MIR
Background:
UniProt Protein Function:MARCH8: E3 ubiquitin-protein ligase that mediates ubiquitination of CD86 and MHC class II proteins, such as HLA-DR alpha and beta, and promotes their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. May also promote ubiquitination and endocytosis of TFRC and FAS.
UniProt Protein Details:

Protein type:EC 6.3.2.-; EC 6.3.2.19; Ligase; Membrane protein, integral; Membrane protein, multi-pass; Ubiquitin conjugating system; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 10q11.21-q11.22

Cellular Component: endosome; lysosome

Molecular Function:ubiquitin-protein ligase activity

Biological Process: protein polyubiquitination

NCBI Summary:MARCH8 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments. MARCH8 induces the internalization of several membrane glycoproteins (Goto et al., 2003 [PubMed 12582153]; Bartee et al., 2004 [PubMed 14722266]).[supplied by OMIM, Apr 2010]
UniProt Code:Q5T0T0
NCBI GenInfo Identifier:74744352
NCBI Gene ID:220972
NCBI Accession:Q5T0T0.1
UniProt Secondary Accession:Q5T0T0,Q5T0S8, Q8TC72, B2R8E7, H0Y7C6,
UniProt Related Accession:Q5T0T0
Molecular Weight:33kDa
NCBI Full Name:E3 ubiquitin-protein ligase MARCH8
NCBI Synonym Full Names:membrane associated ring-CH-type finger 8
NCBI Official Symbol:MARCH8  
NCBI Official Synonym Symbols:MIR; CMIR; c-MIR; RNF178; MARCH-VIII  
NCBI Protein Information:E3 ubiquitin-protein ligase MARCH8
UniProt Protein Name:E3 ubiquitin-protein ligase MARCH8
UniProt Synonym Protein Names:Cellular modulator of immune recognition; c-MIR; Membrane-associated RING finger protein 8; Membrane-associated RING-CH protein VIII; MARCH-VIII; RING finger protein 178; RING-type E3 ubiquitin transferase MARCH8Curated
UniProt Gene Name:MARCH8  
Anti-MARCH8 Antibody (CAB12962)Western blot analysis of extracts of various cell lines, using MARCH8 antibody (CAB12962) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
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