Signal Transduction Antibodies 2

Anti-MARCH6 Antibody (CAB16096)

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SKU:
CAB16096
Product Type:
Antibody
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction

Description

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Antibody Name:Anti-MARCH6 Antibody
Antibody SKU:CAB16096
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 10-80 of human MARCH6 (NP_005876.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Mouse, Rat
Positive Samples:Mouse brain, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 10-80 of human MARCH6 (NP_005876.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:RVCR SEGT PEKP LYHP CVCT GSIK FIHQ ECLV QWLK HSRK EYCE LCKH RFAF TPIY SPDM PSRL PIQD IFA
Gene ID:10299
Uniprot:O60337
Cellular Location:Endoplasmic reticulum membrane, Multi-pass membrane protein
Calculated MW:90kDa/97kDa/102kDa
Observed MW:102kDa
Synonyms:MARCH6, DOA10, MARCH-VI, RNF176, TEB4
Background:This gene encodes a member of a family of membrane-associated E3 ubiquitin ligases containing RING-CH-type zinc finger motifs. Ubiquitination of type II deiodinase by the encoded protein is an important regulatory step in thyroid hormone signalling. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
UniProt Protein Function:MARCH6: E3 ubiquitin-protein ligase that promotes ubiquitination of DIO2, leading to its degradation. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. May cooperate with UBE2G1.
UniProt Protein Details:

Protein type:EC 6.3.2.-; EC 6.3.2.19; Ligase; Membrane protein, integral; Membrane protein, multi-pass; Ubiquitin conjugating system; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 5p15.2

Cellular Component: integral to endoplasmic reticulum membrane; integral to membrane; membrane

Molecular Function:enzyme binding; ubiquitin conjugating enzyme binding; ubiquitin-protein ligase activity

Biological Process: ER-associated protein catabolic process; proteasomal protein catabolic process

NCBI Summary:This gene encodes a member of a family of membrane-associated E3 ubiquitin ligases containing RING-CH-type zinc finger motifs. Ubiquitination of type II deiodinase by the encoded protein is an important regulatory step in thyroid hormone signalling. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]
UniProt Code:O60337
NCBI GenInfo Identifier:2331104
NCBI Gene ID:10299
NCBI Accession:AAB66840.1
UniProt Secondary Accession:O60337,O14670, Q86X77, A5PKZ4, B4DKJ2, B4DT33, D3DTC8
UniProt Related Accession:O60337
Molecular Weight:90,413 Da
NCBI Full Name:TEB4 protein
NCBI Synonym Full Names:membrane associated ring-CH-type finger 6
NCBI Official Symbol:MARCH6  
NCBI Official Synonym Symbols:TEB4; DOA10; RNF176; MARCH-VI  
NCBI Protein Information:E3 ubiquitin-protein ligase MARCH6
UniProt Protein Name:E3 ubiquitin-protein ligase MARCH6
UniProt Synonym Protein Names:Doa10 homolog; Membrane-associated RING finger protein 6; Membrane-associated RING-CH protein VI; MARCH-VI; Protein TEB-4; RING finger protein 176; RING-type E3 ubiquitin transferase MARCH6Curated
UniProt Gene Name:MARCH6  
Anti-MARCH6 Antibody (CAB16096)Western blot analysis of extracts of various cell lines, using MARCH6 Rabbit pAb (CAB16096) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
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