Antibody Name:	Anti-MAPRE2 Antibody
Antibody SKU:	CAB6649
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-327 of human MAPRE2 (NP_001137298.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse kidney, Mouse heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-327 of human MAPRE2 (NP_001137298.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAVN VYST SITQ ETMS RHDI IAWV NDIV SLNY TKVE QLCS GAAY CQFM DMLF PGCI SLKK VKFQ AKLE HEYI HNFK LLQA SFKR MNVD KVIP VEKL VKGR FQDN LDFI QWFK KFYD ANYD GKEY DPVE ARQG QDAI PPPD PGEQ IFNL PKKS HHAN SPTA GAAK SSPA AKPG STPS RPSS AKRA SSSG SASK SDKD LETQ VIQL NEQV HSLK LALE GVEK ERDF YFGK LREI ELLC QEHG QEND DLVQ RLMD ILYA SEEH EGHT EEPE AEEQ AHEQ QPPQ QEEY
Gene ID:	10982
Uniprot:	Q15555
Cellular Location:	Cytoplasm, cytoskeleton
Calculated MW:	29kDa/30kDa/32kDa/35kDa/37kDa
Observed MW:	32KDa

Synonyms:

MAPRE2, CSCSC2, EB1, EB2, RP1

Background:

The protein encoded by this gene shares significant homology to the adenomatous polyposis coli (APC) protein-binding EB1 gene family. This protein is a microtubule-associated protein that is necessary for spindle symmetry during mitosis. It is thought to play a role in the tumorigenesis of colorectal cancers and the proliferative control of normal cells. Alternative splicing of this gene results in multiple transcript variants.

UniProt Protein Function:	RP1: shares significant homology to the adenomatous polyposis coli (APC) protein-binding EB1 gene family. Its function is unknown; however, its homology suggests involvement in tumorigenesis of colorectal cancers and proliferative control of normal cells. This gene may belong to the intermediate/early gene family, involved in the signal transduction cascade downstream of the TCR.
UniProt Protein Details:	Protein type:Cytoskeletal Chromosomal Location of Human Ortholog: 18q12.1 Cellular Component: microtubule cytoskeleton; cytoplasm Molecular Function:microtubule binding Biological Process: mitosis; cell proliferation; cell division; signal transduction
NCBI Summary:	The protein encoded by this gene shares significant homology to the adenomatous polyposis coli (APC) protein-binding EB1 gene family. This protein is a microtubule-associated protein that is necessary for spindle symmetry during mitosis. It is thought to play a role in the tumorigenesis of colorectal cancers and the proliferative control of normal cells. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2012]
UniProt Code:	Q15555
NCBI GenInfo Identifier:	60390165
NCBI Gene ID:	10982
NCBI Accession:	Q15555.1
UniProt Secondary Accession:	Q15555,Q9UQ33, B2RE21, B3KR39, B4DJV4, B7Z2L3, E9PHR3 F5H1V8, G5E9I6,
UniProt Related Accession:	Q15555
Molecular Weight:	327
NCBI Full Name:	Microtubule-associated protein RP/EB family member 2
NCBI Synonym Full Names:	microtubule-associated protein, RP/EB family, member 2
NCBI Official Symbol:	MAPRE2
NCBI Official Synonym Symbols:	EB1; EB2; RP1
NCBI Protein Information:	microtubule-associated protein RP/EB family member 2; end-binding protein 2; APC-binding protein EB1; APC-binding protein EB2; T-cell activation protein, EB1 family
UniProt Protein Name:	Microtubule-associated protein RP/EB family member 2
UniProt Synonym Protein Names:	APC-binding protein EB2; End-binding protein 2; EB2
Protein Family:	Microtubule-associated protein RP/EB family
UniProt Gene Name:	MAPRE2
UniProt Entry Name:	MARE2_HUMAN

	Western blot analysis of extracts of various cell lines, using MAPRE2 antibody (CAB6649) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of HeLa cells using MAPRE2 antibody (CAB6649). Blue: DAPI for nuclear staining.